Presentation of the HB-1 epitope to CD4+ T-cell clone R3A8 by HLA-DR11
. | . | . | . | IFN-γ production, pg/mL* . | . | ||
|---|---|---|---|---|---|---|---|
| EBV-LCLs . | HLA-DR specificity . | . | . | -Peptide . | +Peptide . | ||
| Donor 10 | DR11+ | DR13+ | DR52+ | < | 92 | ||
| No. 22 | DR11+ | DR13- | DR52+ | < | 89 | ||
| No. 23 | DR11+ | DR13- | DR52+ | < | 196 | ||
| No. 24 | DR11- | DR13+ | DR52+ | < | < | ||
| No. 25 | DR11- | DR13- | DR52+ | < | < | ||
| No. 26 | DR11- | DR13- | DR52+ | < | < | ||
| No. 27 | DR11- | DR13- | DR52+ | < | < | ||
| No. 28 | DR11- | DR13- | DR52- | < | < | ||
| No. 29 | DR11- | DR13- | DR52- | < | < | ||
. | . | . | . | IFN-γ production, pg/mL* . | . | ||
|---|---|---|---|---|---|---|---|
| EBV-LCLs . | HLA-DR specificity . | . | . | -Peptide . | +Peptide . | ||
| Donor 10 | DR11+ | DR13+ | DR52+ | < | 92 | ||
| No. 22 | DR11+ | DR13- | DR52+ | < | 89 | ||
| No. 23 | DR11+ | DR13- | DR52+ | < | 196 | ||
| No. 24 | DR11- | DR13+ | DR52+ | < | < | ||
| No. 25 | DR11- | DR13- | DR52+ | < | < | ||
| No. 26 | DR11- | DR13- | DR52+ | < | < | ||
| No. 27 | DR11- | DR13- | DR52+ | < | < | ||
| No. 28 | DR11- | DR13- | DR52- | < | < | ||
| No. 29 | DR11- | DR13- | DR52- | < | < | ||
EBV-LCLs were incubated for 18 hours with 25 μg/mL HB-14-25 peptide and washed. CD4+ T-cell clone R3A8 was then incubated for 20 hours with 30 000 untreated and peptide-pulsed EBV-LCLs. IFN-γ released in the supernatant was measured by ELISA. < indicates < 40 pg/mL IFN-γ.