Presentation of the HB-1 epitope to CD4+ T-cell clone M3A4 by HLA-DR4
. | . | . | . | . | IFN-γ production, pg/mL* . | . | |
|---|---|---|---|---|---|---|---|
| EBV-LCLs . | . | HLA-DR specificity . | . | . | -Peptide . | +Peptide . | |
| Donor 9 | DR3+ | DR4+ | DR52+ | DR53+ | < | 748 | |
| No. 11 | DR3+ | DR4- | DR52+ | DR53- | < | < | |
| No. 12 | DR3+ | DR4- | DR52+ | DR53- | < | < | |
| No. 13 | DR3+ | DR4- | DR52- | DR53- | < | < | |
| No. 14 | DR3— | DR4+ | DR52- | DR53+ | < | 895 | |
| No. 15 | DR3— | DR4+ | DR52- | DR53+ | < | 544 | |
| No. 16 | DR3— | DR4- | DR52+ | DR53+ | < | < | |
| No. 17 | DR3— | DR4- | DR52+ | DR53- | < | < | |
| No. 18 | DR3— | DR4- | DR52+ | DR53- | < | < | |
| No. 19 | DR3— | DR4- | DR52- | DR53+ | < | < | |
| No. 20 | DR3— | DR4- | DR52- | DR53+ | < | < | |
| No. 21 | DR3— | DR4- | DR52- | DR53+ | < | < | |
. | . | . | . | . | IFN-γ production, pg/mL* . | . | |
|---|---|---|---|---|---|---|---|
| EBV-LCLs . | . | HLA-DR specificity . | . | . | -Peptide . | +Peptide . | |
| Donor 9 | DR3+ | DR4+ | DR52+ | DR53+ | < | 748 | |
| No. 11 | DR3+ | DR4- | DR52+ | DR53- | < | < | |
| No. 12 | DR3+ | DR4- | DR52+ | DR53- | < | < | |
| No. 13 | DR3+ | DR4- | DR52- | DR53- | < | < | |
| No. 14 | DR3— | DR4+ | DR52- | DR53+ | < | 895 | |
| No. 15 | DR3— | DR4+ | DR52- | DR53+ | < | 544 | |
| No. 16 | DR3— | DR4- | DR52+ | DR53+ | < | < | |
| No. 17 | DR3— | DR4- | DR52+ | DR53- | < | < | |
| No. 18 | DR3— | DR4- | DR52+ | DR53- | < | < | |
| No. 19 | DR3— | DR4- | DR52- | DR53+ | < | < | |
| No. 20 | DR3— | DR4- | DR52- | DR53+ | < | < | |
| No. 21 | DR3— | DR4- | DR52- | DR53+ | < | < | |
EBV-LCLs were incubated for 18 hours with 25 μg/mL HB-119-41 peptide and washed. CD4+ T-cell clone M3A4 was then incubated for 20 hours with 30 000 untreated and peptide-pulsed EBV-LCLs. IFN-γ released in the supernatant was measured by ELISA. < indicates < 70 pg/mL IFN-γ.