Table 1.

HLA-B44–restricted and HB-1H peptide-specific cytotoxicity of in vitro–induced CD8+ T-cell lines

% Specific lysis‡
CTL donor line
E/T ratio 30: 1
E/T ratio 10: 1
E/T ratio 3: 1
B44 subtype*
HB-1 typing†
+HB-1H
-HB1-H
+HB-1H
-HB1-H
+HB-1H
-HB1-H
1   B*4402   HH   40   4   22   1   11   1  
2   B*4402   HY   35   1   26   1   17   1  
3   B*4402   HH   24   8   22   2   13   1  
4   B*4402   HH   24   10   17   7   15   4  
5   B*4402   HH   4   2   2   2   2   2  
6   B*4403   HH   35   1   21   1   14   1  
7   B*4403   HY   22   13   20   3   13   2  
8
 		 B*4403
 		 HH
 		 2
 		 2
 		 3
 		 2
 		 2
 		 3
 
% Specific lysis‡
CTL donor line
E/T ratio 30: 1
E/T ratio 10: 1
E/T ratio 3: 1
B44 subtype*
HB-1 typing†
+HB-1H
-HB1-H
+HB-1H
-HB1-H
+HB-1H
-HB1-H
1   B*4402   HH   40   4   22   1   11   1  
2   B*4402   HY   35   1   26   1   17   1  
3   B*4402   HH   24   8   22   2   13   1  
4   B*4402   HH   24   10   17   7   15   4  
5   B*4402   HH   4   2   2   2   2   2  
6   B*4403   HH   35   1   21   1   14   1  
7   B*4403   HY   22   13   20   3   13   2  
8
 		 B*4403
 		 HH
 		 2
 		 2
 		 3
 		 2
 		 2
 		 3
 
*

HLA-B44 subtyping was performed by PCR and digestion analysis as described previously.13 

†

HB-1 typing was performed by PCR amplification and digestion of PCR products with restriction enzyme N/aIII, as described previously.15 

‡

Cytolytic activity of HB-1H peptide-induced CTLs was tested against Raji.B*4402 or Raji.B*4403 cells (according to the HLA-B44 subtype of the donor) either untreated or loaded with 5 μM HB-1H.B44 peptide. Specific lysis was determined by chromium release assays at different effector-to-target cell (E/T) ratios.

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