Primer combinations used for amplification
Sense primer . | Antisense primer . | Amplified region . | Amplified ABO alleles (ABO*) . |
|---|---|---|---|
| ABO296s | ABO297as | Enhancer (CBF/NF-Y) | All |
| ABO217s | ABO274as | Promoter, exon 1 | All |
| ABO003s | ABO007as | Exon 2-exon 3 | All |
| ABO003s | ABO127as | Exon 2-intron 2 | All except for O02, O06, O12, O40 |
| ABO003s | ABO128as | Exon 2-intron 2 | O02, O06, O12, O40 |
| ABO212s | ABO012as | Intron 2-exon 4 | O03, Aw08 |
| ABO129s | ABO012as | Exon 3-exon 4 | All |
| ABO205s | ABO012as | Exon 3-exon 4 | O01, O011, O26, O40, O03, Aw08 |
| ABO164s | ABO012as | Intron 3-exon 4 | O02, O06, O12, O40 |
| ABO137s | ABO017as | Exon 4-exon 5 | All |
| ABO130s | ABO214as | Intron 4 | O01, O011, O26 |
| ABO131s | ABO017as | Intron 4-exon 5 | O02, O06, O12, O40 |
| ABO184s | ABO017as | Exon 5 | O02, O06, O12, O39, O03, Aw08 |
| ABO026s | ABO024as | Exon 5-intron 5 | All |
| ABO026s | ABO270as | Exon 5-intron 5 | O01, O011, O26, O21, O02, O06, O12, O39, O40 |
| ABO269s | ABO270as | Exon 5-intron 5 | O01, O011, O26 |
| ABO020s | ABO118as | Exon 6-exon 7 | All |
| ABO023s | ABO118as | Exon 6-exon 7 | A101, A201, Aw04, Aw06, Aw07, Ael01, Ael03, Ax08, B101, Bw07, Bw08, B(A)03, O03, Aw08, O41 |
| ABO021s | ABO118as | Exon 6-exon 7 | O01, O011, O26, O21, O02, O06, O12, O39, O40 |
| ABO028s | ABO118as | Exon 6-exon 7 | A101, A201, Aw04, Aw06, Aw07, Ael01, Ael03, Ax08, O41, O01, O011, O26, O21, O06 |
Sense primer . | Antisense primer . | Amplified region . | Amplified ABO alleles (ABO*) . |
|---|---|---|---|
| ABO296s | ABO297as | Enhancer (CBF/NF-Y) | All |
| ABO217s | ABO274as | Promoter, exon 1 | All |
| ABO003s | ABO007as | Exon 2-exon 3 | All |
| ABO003s | ABO127as | Exon 2-intron 2 | All except for O02, O06, O12, O40 |
| ABO003s | ABO128as | Exon 2-intron 2 | O02, O06, O12, O40 |
| ABO212s | ABO012as | Intron 2-exon 4 | O03, Aw08 |
| ABO129s | ABO012as | Exon 3-exon 4 | All |
| ABO205s | ABO012as | Exon 3-exon 4 | O01, O011, O26, O40, O03, Aw08 |
| ABO164s | ABO012as | Intron 3-exon 4 | O02, O06, O12, O40 |
| ABO137s | ABO017as | Exon 4-exon 5 | All |
| ABO130s | ABO214as | Intron 4 | O01, O011, O26 |
| ABO131s | ABO017as | Intron 4-exon 5 | O02, O06, O12, O40 |
| ABO184s | ABO017as | Exon 5 | O02, O06, O12, O39, O03, Aw08 |
| ABO026s | ABO024as | Exon 5-intron 5 | All |
| ABO026s | ABO270as | Exon 5-intron 5 | O01, O011, O26, O21, O02, O06, O12, O39, O40 |
| ABO269s | ABO270as | Exon 5-intron 5 | O01, O011, O26 |
| ABO020s | ABO118as | Exon 6-exon 7 | All |
| ABO023s | ABO118as | Exon 6-exon 7 | A101, A201, Aw04, Aw06, Aw07, Ael01, Ael03, Ax08, B101, Bw07, Bw08, B(A)03, O03, Aw08, O41 |
| ABO021s | ABO118as | Exon 6-exon 7 | O01, O011, O26, O21, O02, O06, O12, O39, O40 |
| ABO028s | ABO118as | Exon 6-exon 7 | A101, A201, Aw04, Aw06, Aw07, Ael01, Ael03, Ax08, O41, O01, O011, O26, O21, O06 |