Amplification primers
Name . | Sequence (5′→3′) . | Length, bp . | Location . |
|---|---|---|---|
| ABO003s | gatctggactgggtttggag | 20 | Intron 1, nt 12797 to 12816 |
| ABO007as | atgcagcagaagtgatgctg | 20 | Intron 3, nt 1059 to 1078 |
| ABO012as | ggtcaaaactgaagctccag | 20 | Intron 4, nt 194 to 213 |
| ABO017as | tgggcctctgaattcagatg | 20 | Intron 5, nt 129 to 148 |
| ABO020s* | gcacgcctctctccatgtg | 19 | Intron 5, nt 532 to 551 |
| ABO021s* | GGAAGGATGTCCTCGTGGTA | 20 | Exon 6, nt 242 to 261 |
| ABO023s* | GGAAGGATGTCCTCGAGGTG | 20 | Exon 6, nt 242 to 261 |
| ABO024as | ACAATGGGAGCCAGCCAAG | 19 | Exon 6, nt 266 to 284 |
| ABO026s | gctgaatcagagactctgag | 20 | Intron 4, nt 1473 to 1492 |
| ABO028s* | CATTGTCTGGGAGGGCACA | 19 | Exon 6, nt 279 to 297 |
| ABO080as* | AGAAATCGCCCTCGTGCTTA | 20 | Exon 7, nt 771 to 790 |
| ABO090as* | CCGACCCCCCGAAGAACC | 18 | Exon 7, nt 803 to 820 |
| ABO118as | cctaggcttcagttactcac | 20 | 3′-UTR, nt 103 to 122 |
| ABO127as | GACTTCTGGGGCTTAGGAC | 19 | Exon 3, nt 106 to 124 |
| ABO128as | AGACTTCTGGGGCTTAGGAA | 20 | Exon 3, nt 106 to 125 |
| ABO129s | gagctttgctgagaacttgc | 20 | Intron 2, nt 564 to 583 |
| ABO130s | AACCTGACCATCTGCAGCG | 19 | Exon 4, nt 170 to 188 |
| ABO131s | GAACCTGACCATCTGGAGCA | 20 | Exon 4, nt 169 to 188 |
| ABO137s | gcacttgtgccctaaatcct | 20 | Intron 3, nt 1390 to 1409 |
| ABO164s | tcctttctgcagTTACGGGT | 20 | Intron 2, nt 713 to exon 3 nt 106 |
| ABO184s | cttagtccctcttgccagttta | 22 | Intron 4, nt 1446 to 1467 |
| ABO205s | accctcctgcccacgtcca | 19 | Intron 2, nt 521 to 539 |
| ABO212s | cagggtcctaaggacagttaa | 21 | Intron 2, nt 107 to 127 |
| ABO214as | ggtagaccatcctgctagcg | 20 | Intron 4, nt 1678 to 1697 |
| ABO217s* | ggcgccgtcccttcctag | 18 | 5′-UTR, nt -188 to -171 |
| ABO269s | tcacgtggcggcgctttgc | 19 | Intron 4, nt 1660 to 1678 |
| ABO270as | GGGAGCCAGCCAAGGGGTA | 19 | Exon 6, nt 261 to 279 |
| ABO274as* | cctgcggtagcggctccct | 19 | Intron 1, nt 33 to 51 |
| ABO296s* | ggaaacaaatcctacccctac | 21 | 5′-UTR, nt -3988 to -3968 |
| ABO297as* | gtgctgcctgtgcctgttac | 20 | 5′-UTR, nt -3664 to -3645 |
Name . | Sequence (5′→3′) . | Length, bp . | Location . |
|---|---|---|---|
| ABO003s | gatctggactgggtttggag | 20 | Intron 1, nt 12797 to 12816 |
| ABO007as | atgcagcagaagtgatgctg | 20 | Intron 3, nt 1059 to 1078 |
| ABO012as | ggtcaaaactgaagctccag | 20 | Intron 4, nt 194 to 213 |
| ABO017as | tgggcctctgaattcagatg | 20 | Intron 5, nt 129 to 148 |
| ABO020s* | gcacgcctctctccatgtg | 19 | Intron 5, nt 532 to 551 |
| ABO021s* | GGAAGGATGTCCTCGTGGTA | 20 | Exon 6, nt 242 to 261 |
| ABO023s* | GGAAGGATGTCCTCGAGGTG | 20 | Exon 6, nt 242 to 261 |
| ABO024as | ACAATGGGAGCCAGCCAAG | 19 | Exon 6, nt 266 to 284 |
| ABO026s | gctgaatcagagactctgag | 20 | Intron 4, nt 1473 to 1492 |
| ABO028s* | CATTGTCTGGGAGGGCACA | 19 | Exon 6, nt 279 to 297 |
| ABO080as* | AGAAATCGCCCTCGTGCTTA | 20 | Exon 7, nt 771 to 790 |
| ABO090as* | CCGACCCCCCGAAGAACC | 18 | Exon 7, nt 803 to 820 |
| ABO118as | cctaggcttcagttactcac | 20 | 3′-UTR, nt 103 to 122 |
| ABO127as | GACTTCTGGGGCTTAGGAC | 19 | Exon 3, nt 106 to 124 |
| ABO128as | AGACTTCTGGGGCTTAGGAA | 20 | Exon 3, nt 106 to 125 |
| ABO129s | gagctttgctgagaacttgc | 20 | Intron 2, nt 564 to 583 |
| ABO130s | AACCTGACCATCTGCAGCG | 19 | Exon 4, nt 170 to 188 |
| ABO131s | GAACCTGACCATCTGGAGCA | 20 | Exon 4, nt 169 to 188 |
| ABO137s | gcacttgtgccctaaatcct | 20 | Intron 3, nt 1390 to 1409 |
| ABO164s | tcctttctgcagTTACGGGT | 20 | Intron 2, nt 713 to exon 3 nt 106 |
| ABO184s | cttagtccctcttgccagttta | 22 | Intron 4, nt 1446 to 1467 |
| ABO205s | accctcctgcccacgtcca | 19 | Intron 2, nt 521 to 539 |
| ABO212s | cagggtcctaaggacagttaa | 21 | Intron 2, nt 107 to 127 |
| ABO214as | ggtagaccatcctgctagcg | 20 | Intron 4, nt 1678 to 1697 |
| ABO217s* | ggcgccgtcccttcctag | 18 | 5′-UTR, nt -188 to -171 |
| ABO269s | tcacgtggcggcgctttgc | 19 | Intron 4, nt 1660 to 1678 |
| ABO270as | GGGAGCCAGCCAAGGGGTA | 19 | Exon 6, nt 261 to 279 |
| ABO274as* | cctgcggtagcggctccct | 19 | Intron 1, nt 33 to 51 |
| ABO296s* | ggaaacaaatcctacccctac | 21 | 5′-UTR, nt -3988 to -3968 |
| ABO297as* | gtgctgcctgtgcctgttac | 20 | 5′-UTR, nt -3664 to -3645 |
Primer sequences corresponding to noncoding sequences are shown in lowercase. Bold letters indicate artificial mismatches to increase PCR specificity. Nucleotide (nt) positions refer to the mRNA sequence for primers located in the coding region. Primers in the noncoding regions are numbered starting from the beginning of each intron or untranslated region (UTR). The locations of the ABO296s and ABO297as primers used to amplify the Enhancer (CBF/NF-Y) are indicated relative to the transcription start site. s indicates sense primer; as, antisense primer; and nt, nucleotide.
Previously published primers.6,10,14