Genotypic distributions of progeny from crosses of different BAC transgenic founder lines
. | Tg+Fv+/- × Fv+/- . | . | Tg+Fv-/- × Fv+/- . | . | . | ||
|---|---|---|---|---|---|---|---|
. | Tg+, Fv+, %† . | Tg+, Fv-, %† . | Tg+, Fv+, %† . | Tg+, Fv-, %† . | Level* . | ||
| Expected‡ | 75 | 25 | 50 | 50 | - | ||
| AlbA | 86.1 (118) | 13.8 (19) | 53.2 (58) | 46.8 (51) | 31.7 | ||
| AlbB | 74.2 (26) | 25.7 (9) | 48.1 (37) | 51.9 (40) | 13.8 | ||
| AlbC | 83.8 (26) | 16.1 (5) | 50 (7) | 50 (7) | 46.3 | ||
| AlbD | 78.8 (41) | 21.1 (11) | 48.3 (14) | 51.7 (15) | 7.6 | ||
| AlbE | 58.3 (7) | 41.7 (5) | 41.0 (16) | 59 (23) | 14 | ||
| AlbF | 71.1 (37) | 28.8 (15) | 64.3 (18) | 35.7 (10) | 6.6 | ||
| Pf4A | 88.5 (77) | 11.5 (10) | 56.8 (54) | 43.2 (41) | 3.1 | ||
| Pf4B | 100 (49) | 0 (0) | - | - | - | ||
| Pf4C | 100 (46) | 0 (0) | - | - | - | ||
. | Tg+Fv+/- × Fv+/- . | . | Tg+Fv-/- × Fv+/- . | . | . | ||
|---|---|---|---|---|---|---|---|
. | Tg+, Fv+, %† . | Tg+, Fv-, %† . | Tg+, Fv+, %† . | Tg+, Fv-, %† . | Level* . | ||
| Expected‡ | 75 | 25 | 50 | 50 | - | ||
| AlbA | 86.1 (118) | 13.8 (19) | 53.2 (58) | 46.8 (51) | 31.7 | ||
| AlbB | 74.2 (26) | 25.7 (9) | 48.1 (37) | 51.9 (40) | 13.8 | ||
| AlbC | 83.8 (26) | 16.1 (5) | 50 (7) | 50 (7) | 46.3 | ||
| AlbD | 78.8 (41) | 21.1 (11) | 48.3 (14) | 51.7 (15) | 7.6 | ||
| AlbE | 58.3 (7) | 41.7 (5) | 41.0 (16) | 59 (23) | 14 | ||
| AlbF | 71.1 (37) | 28.8 (15) | 64.3 (18) | 35.7 (10) | 6.6 | ||
| Pf4A | 88.5 (77) | 11.5 (10) | 56.8 (54) | 43.2 (41) | 3.1 | ||
| Pf4B | 100 (49) | 0 (0) | - | - | - | ||
| Pf4C | 100 (46) | 0 (0) | - | - | - | ||
Observed percentages of each genotype are shown; numbers within parentheses represent numbers of mice observed. — indicates not applicable.
The level of Fv expression observed in the plasma and platelet pools of the transgenic Fv-null rescued mice (Tg+ Fv-/-) are shown here for all 6 albumin Fv BAC lines and one Pf4 Fv BAC line. The FV level observed in platelets or plasma of a control Fv+/- mouse, corresponding to one endogenous copy of the normal Fv gene, is arbitrarily defined as 50%.
Fv+/+ and Fv+/- genotypes are combined together as Fv+. Only mice carrying transgene are included for analysis.
Expected percentages of each genotype.