YS SP hematopoietic enrichment
. | Experiment no. 1 . | . | . | Experiment no. 2 . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
. | Yolk sac SP . | Whole yolk sac . | Enrichment . | Yolk sac SP . | Whole yolk sac . | Enrichment . | ||||
| 9.5 dpc | 77-fold | 89-fold | ||||||||
| Cells plated | 205 | 205 | 210 | 66 600 | ||||||
| Colonies formed | 78 | 1 | 34 | 120 | ||||||
| Colonies per cell | 380.4 × 10−3 | 4.9 × 10−3 | 161.9 × 10−3 | 1.8 × 10−3 | ||||||
| 10.5 dpc | 26-fold | 41-fold | ||||||||
| Cells plated | 1200 | 60 000 | 775 | 59 500 | ||||||
| Colonies formed | 150 | 284 | 134 | 254 | ||||||
| Colonies per cell | 125.8 × 10−3 | 4.7 × 10−3 | 172.9 × 10−3 | 4.2 × 10−3 | ||||||
| 11.5 dpc | 58-fold | 48-fold | ||||||||
| Cells plated | 600 | 60 000 | 1500 | 60 000 | ||||||
| Colonies formed | 62 | 107 | 109 | 90 | ||||||
| Colonies per cell | 103.3 × 10−3 | 1.8 × 10−3 | 72.6 × 10−3 | 1.5 × 10−3 | ||||||
. | Experiment no. 1 . | . | . | Experiment no. 2 . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
. | Yolk sac SP . | Whole yolk sac . | Enrichment . | Yolk sac SP . | Whole yolk sac . | Enrichment . | ||||
| 9.5 dpc | 77-fold | 89-fold | ||||||||
| Cells plated | 205 | 205 | 210 | 66 600 | ||||||
| Colonies formed | 78 | 1 | 34 | 120 | ||||||
| Colonies per cell | 380.4 × 10−3 | 4.9 × 10−3 | 161.9 × 10−3 | 1.8 × 10−3 | ||||||
| 10.5 dpc | 26-fold | 41-fold | ||||||||
| Cells plated | 1200 | 60 000 | 775 | 59 500 | ||||||
| Colonies formed | 150 | 284 | 134 | 254 | ||||||
| Colonies per cell | 125.8 × 10−3 | 4.7 × 10−3 | 172.9 × 10−3 | 4.2 × 10−3 | ||||||
| 11.5 dpc | 58-fold | 48-fold | ||||||||
| Cells plated | 600 | 60 000 | 1500 | 60 000 | ||||||
| Colonies formed | 62 | 107 | 109 | 90 | ||||||
| Colonies per cell | 103.3 × 10−3 | 1.8 × 10−3 | 72.6 × 10−3 | 1.5 × 10−3 | ||||||
The sum of all colonies scored at 14 days was divided by the sum of all cells seeded. This calculation, carried out for each population for each individual experiment, yielded the per cell colony-forming ability of each population. Dividing the per cell colony-forming ability of the SP cells by the per cell colony-forming ability of the unfractionated tissue yielded the fold increase in the frequency of progenitors in the SP over unfractionated tissue. The maximal enrichment possible of SP over whole tissue for individual experiments is determined by calculating the inverse of SP cells as a percentage of total viable cells (ie, SP comprises 1.29% of total viable cells; inverse of 1.29% is 77; maximal enrichment is 77-fold). Each experiment was replicated in sextuplicate.