Relative intensities of D7Z1 and D7Z2 signals on the derivative chromosome and its allelic equivalent
. | D7Z1 . | . | D7Z2 . | . | ||
|---|---|---|---|---|---|---|
| Patient . | norm . | der . | norm . | der . | ||
| 3 | 130.2 (11.3) | 72.2 (9.1) | 283.0 (40.4) | 277.5 (38.5) | ||
| 6 | 303.7 (9.2) | 182.4 (19.3) | 69.7 (8.3) | 72.1 (8.2) | ||
| 7 | 133.0 (14.7) | 46.4 (8.4) | 49.5 (6.9) | 51.8 (7.5) | ||
| 8 | 115.0 (11.1) | 88.6 (5.9) | 48.0 (15.1) | 48.6 (9.3) | ||
| 12 | 80.2 (9.0) | 55.4 (8.7) | 364.5 (74.8) | 364.2 (70.4) | ||
| 13 | 222.6 (16.6) | 168.8 (16.5) | 245.1 (23.0) | 254.3 (29.2) | ||
| 16 | 293.3 (25.1) | 144.6 (8.3) | 293.0 (30.3) | 290.3 (25.0) | ||
| 17 | 231.4 (22.0) | 210.2 (8.6) | 233.0 (19.4) | 246.1 (16.0) | ||
| 20 | 220.7 (15.3) | 133.3 (4.0) | 64.4 (4.0) | 62.8 (2.0) | ||
| 21 | 225.5 (18.9) | 103.1 (12.3) | 177.0 (8.7) | 173.1 (14.8) | ||
| 22 | 76.3 (4.0) | 61.8 (1.6) | 181.9 (16.9) | 182.3 (9.7) | ||
| 23 | 246.8 (22.1) | 118.7 (6.7) | 55.2 (4.3) | 54.8 (5.5) | ||
| 24 | 144.2 (5.1) | 84.1 (4.0) | 74.3 (3.8) | 75.5 (2.0) | ||
| 27 | 50.1 (4.6) | 49.3 (4.7) | 172.3 (19.8) | 179.6 (27.8) | ||
. | D7Z1 . | . | D7Z2 . | . | ||
|---|---|---|---|---|---|---|
| Patient . | norm . | der . | norm . | der . | ||
| 3 | 130.2 (11.3) | 72.2 (9.1) | 283.0 (40.4) | 277.5 (38.5) | ||
| 6 | 303.7 (9.2) | 182.4 (19.3) | 69.7 (8.3) | 72.1 (8.2) | ||
| 7 | 133.0 (14.7) | 46.4 (8.4) | 49.5 (6.9) | 51.8 (7.5) | ||
| 8 | 115.0 (11.1) | 88.6 (5.9) | 48.0 (15.1) | 48.6 (9.3) | ||
| 12 | 80.2 (9.0) | 55.4 (8.7) | 364.5 (74.8) | 364.2 (70.4) | ||
| 13 | 222.6 (16.6) | 168.8 (16.5) | 245.1 (23.0) | 254.3 (29.2) | ||
| 16 | 293.3 (25.1) | 144.6 (8.3) | 293.0 (30.3) | 290.3 (25.0) | ||
| 17 | 231.4 (22.0) | 210.2 (8.6) | 233.0 (19.4) | 246.1 (16.0) | ||
| 20 | 220.7 (15.3) | 133.3 (4.0) | 64.4 (4.0) | 62.8 (2.0) | ||
| 21 | 225.5 (18.9) | 103.1 (12.3) | 177.0 (8.7) | 173.1 (14.8) | ||
| 22 | 76.3 (4.0) | 61.8 (1.6) | 181.9 (16.9) | 182.3 (9.7) | ||
| 23 | 246.8 (22.1) | 118.7 (6.7) | 55.2 (4.3) | 54.8 (5.5) | ||
| 24 | 144.2 (5.1) | 84.1 (4.0) | 74.3 (3.8) | 75.5 (2.0) | ||
| 27 | 50.1 (4.6) | 49.3 (4.7) | 172.3 (19.8) | 179.6 (27.8) | ||
The allelic equivalent of the derivative chromosome for chr7 was determined by comparing the relative intensities of D7Z2 signals within normal and abnormal metaphases. Then the intensities of D7Z1 and D7Z2 on the derivative chromosome and its equivalent normal chr7 were expressed as relative values to those on their homologous chr7, where the latter were set to 100. The mean values calculated from 30 measurements for normal and abnormal metaphases are presented, and the standard deviations are given in parentheses. We were able to obtain the information regarding the signal reduction of D7Z1 from only 14 of the 27 patients because in the remaining cases, there were either no normal metaphases in their samples or the numbers of normal metaphase were too small to make a reliable statistical assessment.
der indicates the derivative chromosome; and norm, the normal chr7 from which the derivative chromosome was generated.