Relative intensities of D1Z7 and Satlll signals on the derivative chromosome and its allelic equivalent
. | D1Z7 . | . | . | Satlll . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| . | Normal cells . | Tumor cells . | . | Normal cells . | Tumor cells . | . | ||||
| Patient . | chr1* . | chr1* . | der . | chr1* . | chr1* . | der . | ||||
| 1 | ND | 124.9 (18.5) | 3.8 (2.6) | ND | 166.6 (64.2) | 168.2 (59.5) | ||||
| 2 | ND | 80.8 (9.5) | 17.5 (5.5) | ND | 50.2 (17.4) | 48.9 (11.5) | ||||
| 3 | 190.0 (27.3) | 188.3 (24.5) | 37.9 (10.7) | 57.9 (8.8) | 57.2 (10.8) | 56.0 (10.3) | ||||
| 4 | ND | 127.7 (17.6) | 15.9 (7.9) | ND | 41.3 (8.7) | 41.8 (10.2) | ||||
| 5 | ND | 128.0 (19.1) | 7.0 (4.7) | ND | 68.7 (13.3) | 68.3 (12.6) | ||||
| 6 | 68.5 (9.2) | 64.3 (7.8) | 5.7 (3.3) | 56.3 (9.0) | 51.5 (8.4) | 53.7 (11.4) | ||||
| 7 | 163.4 (24.5) | 158.2 (39.0) | 28.8 (14.7) | 46.7 (5.3) | 45.9 (9.2) | 46.1 (7.6) | ||||
| 8 | 225.5 (46.9) | 233.8 (57.2) | 104.9 (37.0) | 129.9 (23.4) | 131.9 (25.4) | 130.1 (22.0) | ||||
| 9 | ND | 75.6 (8.3) | 43.5 (7.0) | ND | 65.0 (4.8) | 69.0 (6.3) | ||||
| 10 | ND | 191.6 (41.2) | 31.3 (12.9) | ND | 48.1 (6.6) | 48.9 (7.7) | ||||
| 11 | ND | 64.3 (9.6) | 37.4 (8.9) | ND | 266.5 (59) | 265.6 (52.2) | ||||
| 12 | 124.7 (14.8) | 122.2 (14.5) | 15.6 (4.2) | 52.3 (6.5) | 49.5 (6.5) | 49.0 (8.7) | ||||
| 13 | 152.6 (15.8) | 149.2 (12.4) | 51.7 (9.3) | 171.5 (24.4) | 174.1 (30.6) | 176.1 (30.5) | ||||
| 14 | ND | 140.2 (13.1) | 50.7 (7.7) | ND | 21.2 (4.6) | 19.3 (7.1) | ||||
| 15 | ND | 149.5 (13.3) | 56.6 (9.7) | ND | 269.3 (43.3) | 270.1 (38.0) | ||||
| 16 | 78.2 (6.1) | 78.1 (8.4) | 33.3 (8.3) | 167.5 (11.6) | 169.3 (18.5) | 170.4 (18.1) | ||||
| 17 | 130.8 (9.5) | 129.2 (9.8) | 8.4 (2.7) | 75.2 (6.5) | 72.1 (7.1) | 72.1 (6.8) | ||||
| 18 | ND | 70.7 (7.1) | 10.2 (7.0) | ND | 63.2 (8.2) | 63.2 (8.0) | ||||
| 19 | ND | 149.7 (14.9) | 24.8 (6.6) | ND | 60.9 (10.3) | 62.1 (9.8) | ||||
| 20 | 64.7 (9.1) | 65.3 (6.5) | 33.4 (3.7) | 45.3 (7.0) | 43.4 (6.5) | 43.1 (8.4) | ||||
| 21 | 153.6 (8.7) | 158.5 (18.1) | 69.1 (10.0) | 51.7 (4.2) | 50.8 (10.0) | 50.8 (9.3) | ||||
| 22 | 149.4 (10.8) | 143.4 (19.0) | 31.3 (12.6) | 20.8 (2.8) | 21.7 (3.6) | 20.6 (4.7) | ||||
| 23 | 185.0 (10.7) | 179.0 (17.5) | 45.3 (13.9) | 138.9 (6.5) | 137.1 (13.1) | 136.7 (17.1) | ||||
| 24 | 137.4 (6.1) | 142.8 (13.0) | 58.5 (14.2) | 34.9 (2.8) | 35.2 (4.4) | 35.8 (6.3) | ||||
| 25 | ND | 160.5 (16.0) | 51.6 (7.9) | ND | 52.9 (7.5) | 52.6 (6.6) | ||||
| 26 | ND | 192.9 (8.7) | 22.7 (6.0) | ND | 22.0 (1.8) | 22.7 (2.7) | ||||
| 27 | 52.9 (4.9) | 53.8 (5.5) | 0 | 45.7 (3.1) | 45.1 (6.1) | 47.4 (6.2) | ||||
. | D1Z7 . | . | . | Satlll . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| . | Normal cells . | Tumor cells . | . | Normal cells . | Tumor cells . | . | ||||
| Patient . | chr1* . | chr1* . | der . | chr1* . | chr1* . | der . | ||||
| 1 | ND | 124.9 (18.5) | 3.8 (2.6) | ND | 166.6 (64.2) | 168.2 (59.5) | ||||
| 2 | ND | 80.8 (9.5) | 17.5 (5.5) | ND | 50.2 (17.4) | 48.9 (11.5) | ||||
| 3 | 190.0 (27.3) | 188.3 (24.5) | 37.9 (10.7) | 57.9 (8.8) | 57.2 (10.8) | 56.0 (10.3) | ||||
| 4 | ND | 127.7 (17.6) | 15.9 (7.9) | ND | 41.3 (8.7) | 41.8 (10.2) | ||||
| 5 | ND | 128.0 (19.1) | 7.0 (4.7) | ND | 68.7 (13.3) | 68.3 (12.6) | ||||
| 6 | 68.5 (9.2) | 64.3 (7.8) | 5.7 (3.3) | 56.3 (9.0) | 51.5 (8.4) | 53.7 (11.4) | ||||
| 7 | 163.4 (24.5) | 158.2 (39.0) | 28.8 (14.7) | 46.7 (5.3) | 45.9 (9.2) | 46.1 (7.6) | ||||
| 8 | 225.5 (46.9) | 233.8 (57.2) | 104.9 (37.0) | 129.9 (23.4) | 131.9 (25.4) | 130.1 (22.0) | ||||
| 9 | ND | 75.6 (8.3) | 43.5 (7.0) | ND | 65.0 (4.8) | 69.0 (6.3) | ||||
| 10 | ND | 191.6 (41.2) | 31.3 (12.9) | ND | 48.1 (6.6) | 48.9 (7.7) | ||||
| 11 | ND | 64.3 (9.6) | 37.4 (8.9) | ND | 266.5 (59) | 265.6 (52.2) | ||||
| 12 | 124.7 (14.8) | 122.2 (14.5) | 15.6 (4.2) | 52.3 (6.5) | 49.5 (6.5) | 49.0 (8.7) | ||||
| 13 | 152.6 (15.8) | 149.2 (12.4) | 51.7 (9.3) | 171.5 (24.4) | 174.1 (30.6) | 176.1 (30.5) | ||||
| 14 | ND | 140.2 (13.1) | 50.7 (7.7) | ND | 21.2 (4.6) | 19.3 (7.1) | ||||
| 15 | ND | 149.5 (13.3) | 56.6 (9.7) | ND | 269.3 (43.3) | 270.1 (38.0) | ||||
| 16 | 78.2 (6.1) | 78.1 (8.4) | 33.3 (8.3) | 167.5 (11.6) | 169.3 (18.5) | 170.4 (18.1) | ||||
| 17 | 130.8 (9.5) | 129.2 (9.8) | 8.4 (2.7) | 75.2 (6.5) | 72.1 (7.1) | 72.1 (6.8) | ||||
| 18 | ND | 70.7 (7.1) | 10.2 (7.0) | ND | 63.2 (8.2) | 63.2 (8.0) | ||||
| 19 | ND | 149.7 (14.9) | 24.8 (6.6) | ND | 60.9 (10.3) | 62.1 (9.8) | ||||
| 20 | 64.7 (9.1) | 65.3 (6.5) | 33.4 (3.7) | 45.3 (7.0) | 43.4 (6.5) | 43.1 (8.4) | ||||
| 21 | 153.6 (8.7) | 158.5 (18.1) | 69.1 (10.0) | 51.7 (4.2) | 50.8 (10.0) | 50.8 (9.3) | ||||
| 22 | 149.4 (10.8) | 143.4 (19.0) | 31.3 (12.6) | 20.8 (2.8) | 21.7 (3.6) | 20.6 (4.7) | ||||
| 23 | 185.0 (10.7) | 179.0 (17.5) | 45.3 (13.9) | 138.9 (6.5) | 137.1 (13.1) | 136.7 (17.1) | ||||
| 24 | 137.4 (6.1) | 142.8 (13.0) | 58.5 (14.2) | 34.9 (2.8) | 35.2 (4.4) | 35.8 (6.3) | ||||
| 25 | ND | 160.5 (16.0) | 51.6 (7.9) | ND | 52.9 (7.5) | 52.6 (6.6) | ||||
| 26 | ND | 192.9 (8.7) | 22.7 (6.0) | ND | 22.0 (1.8) | 22.7 (2.7) | ||||
| 27 | 52.9 (4.9) | 53.8 (5.5) | 0 | 45.7 (3.1) | 45.1 (6.1) | 47.4 (6.2) | ||||
The allelic equivalent of the derivative chromosome for chr1 was determined by comparing the relative intensities of Satlll signals within normal and abnormal metaphases. Then the intensities of D1Z7 and Satlll on the derivative chromosome and its equivalent normal chr1 were expressed as relative values to those on their homologous chr1, where the latter were set to 100. The mean values calculated from 30 measurements for normal and abnormal metaphases are presented, and the standard deviations are given in parentheses.
der indicates the derivative chromosome; and chr1*, the normal chr1 from which the derivative chromosome was generated. ND indicates that there is either no detectable normal metaphase in the samples or that the normal metaphases are too little to get a reliable statistical estimation.