Table 1. Oligonucleotide sequences of... | American Society of Hematology

Table 1.

Oligonucleotide sequences of primers used in PCR

Application	Amplified fragment, bp*	DNA strand	Oligonucleotides
Screening SMC library	1083-2572	Forward	5′TGAGTTGGAGAGACTGGACC 3′
Screening SMC library	1083-2572	Reverse	5′GAGAGATGTATCACACTAAG 3′
MTE, complete cDNA clone SSH6	743-1292	Forward	5′GGCTCGTAAATTGGATA 3′
MTE, complete cDNA clone SSH6	743-1292	Reverse	5′TTACCTCCATTAGGACGTCC 3′
MTE, unique insert in clone SSH6	1048-1168	Forward	5′GGACTTGCCATGAGGTGTTGAAG 3′
MTE, unique insert in clone SSH6	1048-1168	Reverse	5′CTTTGTTGATGATGGTGGAGTAG 3′
Cloning GST-vasculin	1597-2545	Forward	5′CCTAAATCTAGAGCGTCGACGATGCTGG 3′
Cloning GST-vasculin	1597-2545	Reverse	5′AAGCTGTTAGTCGACCCTTCACA 3′

Application	Amplified fragment, bp*	DNA strand	Oligonucleotides
Screening SMC library	1083-2572	Forward	5′TGAGTTGGAGAGACTGGACC 3′
Screening SMC library	1083-2572	Reverse	5′GAGAGATGTATCACACTAAG 3′
MTE, complete cDNA clone SSH6	743-1292	Forward	5′GGCTCGTAAATTGGATA 3′
MTE, complete cDNA clone SSH6	743-1292	Reverse	5′TTACCTCCATTAGGACGTCC 3′
MTE, unique insert in clone SSH6	1048-1168	Forward	5′GGACTTGCCATGAGGTGTTGAAG 3′
MTE, unique insert in clone SSH6	1048-1168	Reverse	5′CTTTGTTGATGATGGTGGAGTAG 3′
Cloning GST-vasculin	1597-2545	Forward	5′CCTAAATCTAGAGCGTCGACGATGCTGG 3′
Cloning GST-vasculin	1597-2545	Reverse	5′AAGCTGTTAGTCGACCCTTCACA 3′

Underlined sequence indicates SalI restriction site; mutated residues are indicated in bold.

Amplified vasculin cDNA fragment, according to position in clone A6.3 (Figure 1).