Factor V activity in platelet pool of control mice and mice that received transplants
. | Whole blood FV activity, mU/mL* . | Platelet FV activity, mU/mL* . | % Platelet FV pool† . | % Platelet FV pool‡ . |
|---|---|---|---|---|
| Wild-type C57BL/6J | ||||
| A | 5015 | 1650 | 33 (10) | — |
| B | 5026 | 3845 | 77 (24) | 16 |
| C | 2788 | 758 | 27 (8) | 26 |
| D | 4797 | 2998 | 63 (19) | 6 |
| E | 5535 | 1212 | 22 (7) | 7 |
| Mean ± SD | 4632 ± 1066 | 2047 ± 1289 | 44 ± 24 (14) | 14 ± 9 |
| Wild-type Fv FLC recipients | ||||
| F | 3648 | 1893 | 52 (16) | — |
| G | 2102 | 1322 | 63 (20) | — |
| H | 5017 | 2187 | 44 (14) | — |
| I | 5104 | 1199 | 24 (7) | — |
| Mean ± SD | 3968 ± 1411 | 1804 ± 469 | 45 ± 17 (14) | — |
| Fv-null FLC recipients | ||||
| J | 4151 | 10 | 0.2 (<0.1) | — |
| K | 1579 | 20 | 1.3 (0.4) | — |
| M | 2265 | 3 | 0.1 (<0.1) | — |
| O | 4628 | 2 | <0.1 (<0.1) | — |
| P | 2192 | 6 | 0.3 (<0.1) | — |
| Q | 5282 | 5 | 0.1 (<0.1) | — |
| R | 3997 | 1 | <0.1 (<0.1) | — |
| S | 4092 | 9 | 0.2 (<0.1) | — |
| Mean ± SD | 3523 ± 1330 | 10 ± 6 | 0.3 ± 0.4 (0.1) | — |
. | Whole blood FV activity, mU/mL* . | Platelet FV activity, mU/mL* . | % Platelet FV pool† . | % Platelet FV pool‡ . |
|---|---|---|---|---|
| Wild-type C57BL/6J | ||||
| A | 5015 | 1650 | 33 (10) | — |
| B | 5026 | 3845 | 77 (24) | 16 |
| C | 2788 | 758 | 27 (8) | 26 |
| D | 4797 | 2998 | 63 (19) | 6 |
| E | 5535 | 1212 | 22 (7) | 7 |
| Mean ± SD | 4632 ± 1066 | 2047 ± 1289 | 44 ± 24 (14) | 14 ± 9 |
| Wild-type Fv FLC recipients | ||||
| F | 3648 | 1893 | 52 (16) | — |
| G | 2102 | 1322 | 63 (20) | — |
| H | 5017 | 2187 | 44 (14) | — |
| I | 5104 | 1199 | 24 (7) | — |
| Mean ± SD | 3968 ± 1411 | 1804 ± 469 | 45 ± 17 (14) | — |
| Fv-null FLC recipients | ||||
| J | 4151 | 10 | 0.2 (<0.1) | — |
| K | 1579 | 20 | 1.3 (0.4) | — |
| M | 2265 | 3 | 0.1 (<0.1) | — |
| O | 4628 | 2 | <0.1 (<0.1) | — |
| P | 2192 | 6 | 0.3 (<0.1) | — |
| Q | 5282 | 5 | 0.1 (<0.1) | — |
| R | 3997 | 1 | <0.1 (<0.1) | — |
| S | 4092 | 9 | 0.2 (<0.1) | — |
| Mean ± SD | 3523 ± 1330 | 10 ± 6 | 0.3 ± 0.4 (0.1) | — |
— indicates not done.
FV activity measured with a 1-stage assay in whole blood and in platelets.
Size of the platelet FV pool expressed as percent of the total activity measured in whole blood. Corrected estimates of the platelet FV pool size based on the 2-stage FV assay are shown in parentheses (see “Materials and methods”).
2-stage assay.