Morphometric analysis of Ang-1-mediated cytoskeleton modification and RhoGTPase activation in ECs
Parameter examined . | Unstimulated cells . | Ang-1, 2 min . | Ang-1, 5 min . |
|---|---|---|---|
| Lamellipodia, % positive cells*† | 10.0 ± 0.2 | 75.3 ± 1.2 | Not tested |
| GST-PBD fluorescence of lamellipodia-positive cells, arbitrary unit‡ | 15.6 ± 3.1 | 37.5 ± 5.5 | Not tested |
| F-actin cables, % positive cells†§ | 7.8 ± 2.1 | Not tested | 72.5 ± 2.3 |
| GST-RBD fluorescence of F-actin cable-positive cells, arbitrary unit∥ | 12.6 ± 3 | Not tested | 31.5 ± 2.2 |
Parameter examined . | Unstimulated cells . | Ang-1, 2 min . | Ang-1, 5 min . |
|---|---|---|---|
| Lamellipodia, % positive cells*† | 10.0 ± 0.2 | 75.3 ± 1.2 | Not tested |
| GST-PBD fluorescence of lamellipodia-positive cells, arbitrary unit‡ | 15.6 ± 3.1 | 37.5 ± 5.5 | Not tested |
| F-actin cables, % positive cells†§ | 7.8 ± 2.1 | Not tested | 72.5 ± 2.3 |
| GST-RBD fluorescence of F-actin cable-positive cells, arbitrary unit∥ | 12.6 ± 3 | Not tested | 31.5 ± 2.2 |
Lamellipodia were identified according to Nobes and Hall15 in fixed and permeabilized cells stained with FITC-phalloidin.
Mean ± SD of 3 individual experiments. For each experiment, 100 cells were counted.
The results are expressed as mean ± SD of 30 lamellipodia-positive cells in 3 different experiments.
Cells were fixed, permeabilized, and stained by FITC-phalloidin. A fluorescence intensity greater than 40 arbitrary units was a limit to consider cells positive for the presence of F-actin cables.
The results are expressed as mean ± SD of 30 F-actin cable-positive cells in 3 different experiments.