Table 1. Single-cell PCR analysis of... | American Society of Hematology

Table 1.

Single-cell PCR analysis of human tonsillar IRTA1⁺ cells

Tonsil	Cell phenotype	No. of cells positive/cells analyzed (%)	No. of cells mutated/cells positive for IRTA1 (%)	Average mutation frequency, % (range)^*
1	IRTA1⁺	15/100 (15)	8/15 (53.3)	5.6 (2.6−9.4)
2	IRTA1⁺	21/120 (18)	19/21 (90.5)	7.5 (0.5−15.9)
Controls
1	CD3⁺	0/25	—	—
1	Buffer controls†	0/20	—	—
2	Buffer controls†	0/40	—	—

Tonsil	Cell phenotype	No. of cells positive/cells analyzed (%)	No. of cells mutated/cells positive for IRTA1 (%)	Average mutation frequency, % (range)^*
1	IRTA1⁺	15/100 (15)	8/15 (53.3)	5.6 (2.6−9.4)
2	IRTA1⁺	21/120 (18)	19/21 (90.5)	7.5 (0.5−15.9)
Controls
1	CD3⁺	0/25	—	—
1	Buffer controls†	0/20	—	—
2	Buffer controls†	0/40	—	—

— indicates not applicable.

Considering the mutated V gene rearrangements only.

See “Materials and methods.”