Engraftment of 10 huPBMC donors in RAG2−/− γc−/− mice: proportion of CD45+, CD4/CD8, and CD4+ CD8+ cells in peripheral blood in time
. | Day 14 . | . | . | Day 28 . | . | . | Day 63 . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mice . | hCD45 . | CD4/CD8 . | CD4+ CD8+ . | hCD45 . | CD4/CD8 . | CD4+ CD8+ . | hCD45 . | CD4/CD8 . | CD4+ CD8+ . | ||||||
| 1A | 48.0 | 1.2 | 20.0 | — | — | — | — | — | — | ||||||
| 1B | 35.0 | 1.5 | 18.0 | — | — | — | — | — | — | ||||||
| 1C | 39.0 | 1.4 | 24.0 | 96.0 | 0.3 | 8.0 | — | — | — | ||||||
| 2A | 11.0 | 0.5 | 0 | — | — | 5.0 | — | — | — | ||||||
| 2B | 7.5 | 0.6 | 7.0 | — | — | 14.0 | — | — | — | ||||||
| 2C | 1.1 | 1.0 | 0 | — | — | 10.0 | — | — | — | ||||||
| 3A | — | — | — | — | — | — | — | — | — | ||||||
| 3B | 84.5 | 0.4 | 9.0 | — | — | — | — | — | — | ||||||
| 3C | 77.0 | 3.4 | 10.0 | — | — | — | — | — | — | ||||||
| 4A | 82.0 | 1.5 | 8.0 | — | — | — | — | — | — | ||||||
| 4B | 93.5 | 1.3 | 9.0 | — | — | — | — | — | — | ||||||
| 4C | — | — | — | — | — | — | — | — | — | ||||||
| 5A | 0.1 | — | 0 | 24.5 | 0.1 | 2.0 | 4.0 | 0.4 | 13.0 | ||||||
| 5B | 0.7 | 0.3 | 0 | 43.5 | 0 | 12.0 | 62.5 | 2.8 | 4.0 | ||||||
| 5C | 18.5 | 0.4 | 10.0 | — | — | — | — | — | — | ||||||
| 6A | 0.3 | 2.0 | 0 | 8.0 | 0.2 | 15.0 | — | — | — | ||||||
| 6B | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||||||
| 6C | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||||||
| 7A | 14.0 | 0.7 | 24.0 | — | — | — | — | — | — | ||||||
| 7B | — | — | — | — | — | — | — | — | — | ||||||
| 7C | 84.5 | 1.0 | 14.0 | — | — | — | — | — | — | ||||||
| 8A | 3.5 | 1.2 | 21.0 | 24.5 | 0.2 | 10.0 | 20.5 | 0.6 | 10.0 | ||||||
| 8B | 6.0 | 1.0 | 13.0 | 14.0 | 0.3 | 16.0 | — | — | — | ||||||
| 8C | 27.5 | 0.9 | 15.0 | 35.0 | 0.2 | 12.0 | — | — | — | ||||||
| 9A | 21.5 | 2.0 | 12.0 | 6.0 | 1.0 | 18.0 | 10.5 | 0.7 | 29.0 | ||||||
| 9B | 55.0 | 2.0 | 14.0 | 17.0 | 1.2 | 20.0 | — | — | — | ||||||
| 9C | 41.5 | 2.4 | 12.0 | 31.0 | 1.8 | 13.0 | — | — | — | ||||||
| 10A | 91.0 | 2.3 | 15.0 | — | — | — | — | — | — | ||||||
| 10B | 73.5 | 0.7 | 26.0 | — | — | — | — | — | — | ||||||
| 10C | — | — | — | — | — | — | — | — | — | ||||||
. | Day 14 . | . | . | Day 28 . | . | . | Day 63 . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mice . | hCD45 . | CD4/CD8 . | CD4+ CD8+ . | hCD45 . | CD4/CD8 . | CD4+ CD8+ . | hCD45 . | CD4/CD8 . | CD4+ CD8+ . | ||||||
| 1A | 48.0 | 1.2 | 20.0 | — | — | — | — | — | — | ||||||
| 1B | 35.0 | 1.5 | 18.0 | — | — | — | — | — | — | ||||||
| 1C | 39.0 | 1.4 | 24.0 | 96.0 | 0.3 | 8.0 | — | — | — | ||||||
| 2A | 11.0 | 0.5 | 0 | — | — | 5.0 | — | — | — | ||||||
| 2B | 7.5 | 0.6 | 7.0 | — | — | 14.0 | — | — | — | ||||||
| 2C | 1.1 | 1.0 | 0 | — | — | 10.0 | — | — | — | ||||||
| 3A | — | — | — | — | — | — | — | — | — | ||||||
| 3B | 84.5 | 0.4 | 9.0 | — | — | — | — | — | — | ||||||
| 3C | 77.0 | 3.4 | 10.0 | — | — | — | — | — | — | ||||||
| 4A | 82.0 | 1.5 | 8.0 | — | — | — | — | — | — | ||||||
| 4B | 93.5 | 1.3 | 9.0 | — | — | — | — | — | — | ||||||
| 4C | — | — | — | — | — | — | — | — | — | ||||||
| 5A | 0.1 | — | 0 | 24.5 | 0.1 | 2.0 | 4.0 | 0.4 | 13.0 | ||||||
| 5B | 0.7 | 0.3 | 0 | 43.5 | 0 | 12.0 | 62.5 | 2.8 | 4.0 | ||||||
| 5C | 18.5 | 0.4 | 10.0 | — | — | — | — | — | — | ||||||
| 6A | 0.3 | 2.0 | 0 | 8.0 | 0.2 | 15.0 | — | — | — | ||||||
| 6B | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||||||
| 6C | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||||||
| 7A | 14.0 | 0.7 | 24.0 | — | — | — | — | — | — | ||||||
| 7B | — | — | — | — | — | — | — | — | — | ||||||
| 7C | 84.5 | 1.0 | 14.0 | — | — | — | — | — | — | ||||||
| 8A | 3.5 | 1.2 | 21.0 | 24.5 | 0.2 | 10.0 | 20.5 | 0.6 | 10.0 | ||||||
| 8B | 6.0 | 1.0 | 13.0 | 14.0 | 0.3 | 16.0 | — | — | — | ||||||
| 8C | 27.5 | 0.9 | 15.0 | 35.0 | 0.2 | 12.0 | — | — | — | ||||||
| 9A | 21.5 | 2.0 | 12.0 | 6.0 | 1.0 | 18.0 | 10.5 | 0.7 | 29.0 | ||||||
| 9B | 55.0 | 2.0 | 14.0 | 17.0 | 1.2 | 20.0 | — | — | — | ||||||
| 9C | 41.5 | 2.4 | 12.0 | 31.0 | 1.8 | 13.0 | — | — | — | ||||||
| 10A | 91.0 | 2.3 | 15.0 | — | — | — | — | — | — | ||||||
| 10B | 73.5 | 0.7 | 26.0 | — | — | — | — | — | — | ||||||
| 10C | — | — | — | — | — | — | — | — | — | ||||||
HuPBMCs of 10 different donors were injected into 30 mice (3 mice per donor group). Engraftment rates of human cells (%hCD45) as a fraction of total (mouse + human) CD45+ cells, CD4/CD8 ratios, and fractions (%) of CD4+/CD8+ cells in peripheral blood are shown. One mouse (9A) developed chronic X-GVHD with a high fraction of CD4+/CD8+ cells late in time.
— indicates death of mice without further data.