scuPA kringle and linker mutants
Kringle mutants | |
| Wild-type-scuPA | 102-C R N P D N R R R P W C-112 |
| scuPA-Lys 105-110Ala | 102-C R N A A A A A A P W C-112 |
| scuPA-Lys 107-109Ala | 102-C R N P D A A A R P W C-112 |
| scuPA-Lys 108-110Ala | 102-C R N P D N A A A P W C-112 |
| scuPA-Lys 110Ala | 102-C R N P D N R R A P W C-112 |
| scuPA-Lys 107Ala | 102-C R N P D A R R R P W C-112 |
| Linker mutants | |
| Wild-type-scuPA | 42-C E I D K S K T C-50 |
| scuPA-Leu43-49Ala | 42-C A A A A A A A C-50 |
| ΔLeu45-48-scuPA | 42-C - - - - - - - - C-50 |
| scuPA-Lys48Pro | 42-C E I D K S P T C-50 |
| ScuPA-Ser47Gly | 42-C E I D K G K T C-50 |
Kringle mutants | |
| Wild-type-scuPA | 102-C R N P D N R R R P W C-112 |
| scuPA-Lys 105-110Ala | 102-C R N A A A A A A P W C-112 |
| scuPA-Lys 107-109Ala | 102-C R N P D A A A R P W C-112 |
| scuPA-Lys 108-110Ala | 102-C R N P D N A A A P W C-112 |
| scuPA-Lys 110Ala | 102-C R N P D N R R A P W C-112 |
| scuPA-Lys 107Ala | 102-C R N P D A R R R P W C-112 |
| Linker mutants | |
| Wild-type-scuPA | 42-C E I D K S K T C-50 |
| scuPA-Leu43-49Ala | 42-C A A A A A A A C-50 |
| ΔLeu45-48-scuPA | 42-C - - - - - - - - C-50 |
| scuPA-Lys48Pro | 42-C E I D K S P T C-50 |
| ScuPA-Ser47Gly | 42-C E I D K G K T C-50 |
Amino acids in the heparin binding site of uPA kringle (residues 105-110) and in the linker region (residues 43-49) between the growth factor domain and the kringle were either mutated to the indicated amino acids (bold) or deleted (-).