Laboratory measurements of the hemophilia B dog G11 after administration of HD vector AdFTC/cFIX/ChMAR
Time, d* . | Alk phos level, U/L . | Bilirubin level, mg/dL . | GGTP level, U/L . | PLT counts, 103/mm3* . | aPTT level, s* . | % FIX activity % NCP* . | WBC count, 103/mm3 . | HCT level, % . | HGB level g/dL . | CPK level, U/L . | Urea nitrogen level, mg/dL . | Creat level, mg/dL . | Total protein level, g/dL . | Albumin level, g/dL . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| −2 | 222 | 0.1 | < 5 | 325 | > 150 | ND | 16.4 | 32.6 | 10.8 | 154 | 28 | 0.8 | 4.9 | 3 |
| −1 | 228 | 0.2 | < 5 | 281 | > 150 | < 1 | 17.6H | 31.7 | 10.2 | 374 | 34 | 0.8 | 5 | 3 |
| 0 | ND | ND | ND | 295 | ND | ND | 7.6 | 31.3 | 10.5 | ND | ND | ND | ND | ND |
| 0.48 | 204 | 0.1 | < 5 | 284 | > 150 | < 1 | 11.9 | 28.9 | 10 | 316 | 26 | 0.7 | 4.7 | 2.7 |
| 1 | 208 | 0.1 | < 5 | 261 | 131.9 | 9.4 | 14.2 | 31.1 | 9.9 | 420 | 32 | 0.8 | 4.7 | 2.9 |
| 2 | 227 | 0.1 | < 5 | 297 | 84.3 | 36.5 | 17.5H | 31.0 | 10.3 | 520 | 32 | 0.8 | 4.7 | 2.9 |
| 3 | 249 | 0.1 | < 5 | 253 | 86.4 | 58.5 | 17.9H | 29.9 | 10.3 | 407 | 24 | 0.6 | 4.7 | 3 |
| 6 | 218 | 0.1 | < 5 | 269 | ND | ND | 16.3 | 30.6 | 10.1 | 272 | 27 | 0.9 | 4.7 | 2.9 |
| 8 | 211 | 0.1 | < 5 | 173L | ND | ND | 16.6 | 31.6 | 10.4 | 492 | 30 | 1 | 4.8 | 2.9 |
| 10 | 545 | 0.1 | < 5 | 180L | ND | ND | 21.0H | 34.6 | 11.4 | 594 | 28 | 0.7 | 5.4 | 2.8 |
| 13 | ND | ND | ND | 268 | ND | ND | 19.4H | 33.1 | 10.9 | ND | ND | ND | ND | ND |
| 15 | ND | ND | ND | 260 | ND | ND | 15 | 33.4 | 11 | ND | ND | ND | ND | ND |
| 17 | ND | ND | ND | 322 | ND | ND | 16.3 | 32.8 | 10.5 | ND | ND | ND | ND | ND |
| 20 | ND | ND | ND | 397 | ND | ND | 18.2H | 31 | 10.2 | ND | ND | ND | ND | ND |
| 22 | ND | ND | ND | 392 | ND | ND | 18.9H | 34.4 | 11.4 | ND | ND | ND | ND | ND |
| 31 | ND | ND | ND | 408 | ND | ND | 15.4 | 36.1 | 12.1 | ND | ND | ND | ND | ND |
| 42 | ND | ND | ND | 399 | ND | ND | 16.4 | 41.2 | 13.3 | ND | ND | ND | ND | ND |
| 49 | ND | ND | ND | 398 | ND | ND | 16.4 | 43 | 14.2 | ND | ND | ND | ND | ND |
| 56 | ND | ND | ND | 362 | ND | ND | 16.5 | 40.5 | 13.5 | ND | ND | ND | ND | ND |
| 63 | ND | ND | ND | 266 | ND | ND | 16.4 | 43.9 | 14.3 | ND | ND | ND | ND | ND |
| 121 | 156 | 0.1 | < 5 | 303 | ND | ND | 11.6 | 52.9 | 18 | 159 | 41 | 1 | 5.5 | 3.4 |
Time, d* . | Alk phos level, U/L . | Bilirubin level, mg/dL . | GGTP level, U/L . | PLT counts, 103/mm3* . | aPTT level, s* . | % FIX activity % NCP* . | WBC count, 103/mm3 . | HCT level, % . | HGB level g/dL . | CPK level, U/L . | Urea nitrogen level, mg/dL . | Creat level, mg/dL . | Total protein level, g/dL . | Albumin level, g/dL . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| −2 | 222 | 0.1 | < 5 | 325 | > 150 | ND | 16.4 | 32.6 | 10.8 | 154 | 28 | 0.8 | 4.9 | 3 |
| −1 | 228 | 0.2 | < 5 | 281 | > 150 | < 1 | 17.6H | 31.7 | 10.2 | 374 | 34 | 0.8 | 5 | 3 |
| 0 | ND | ND | ND | 295 | ND | ND | 7.6 | 31.3 | 10.5 | ND | ND | ND | ND | ND |
| 0.48 | 204 | 0.1 | < 5 | 284 | > 150 | < 1 | 11.9 | 28.9 | 10 | 316 | 26 | 0.7 | 4.7 | 2.7 |
| 1 | 208 | 0.1 | < 5 | 261 | 131.9 | 9.4 | 14.2 | 31.1 | 9.9 | 420 | 32 | 0.8 | 4.7 | 2.9 |
| 2 | 227 | 0.1 | < 5 | 297 | 84.3 | 36.5 | 17.5H | 31.0 | 10.3 | 520 | 32 | 0.8 | 4.7 | 2.9 |
| 3 | 249 | 0.1 | < 5 | 253 | 86.4 | 58.5 | 17.9H | 29.9 | 10.3 | 407 | 24 | 0.6 | 4.7 | 3 |
| 6 | 218 | 0.1 | < 5 | 269 | ND | ND | 16.3 | 30.6 | 10.1 | 272 | 27 | 0.9 | 4.7 | 2.9 |
| 8 | 211 | 0.1 | < 5 | 173L | ND | ND | 16.6 | 31.6 | 10.4 | 492 | 30 | 1 | 4.8 | 2.9 |
| 10 | 545 | 0.1 | < 5 | 180L | ND | ND | 21.0H | 34.6 | 11.4 | 594 | 28 | 0.7 | 5.4 | 2.8 |
| 13 | ND | ND | ND | 268 | ND | ND | 19.4H | 33.1 | 10.9 | ND | ND | ND | ND | ND |
| 15 | ND | ND | ND | 260 | ND | ND | 15 | 33.4 | 11 | ND | ND | ND | ND | ND |
| 17 | ND | ND | ND | 322 | ND | ND | 16.3 | 32.8 | 10.5 | ND | ND | ND | ND | ND |
| 20 | ND | ND | ND | 397 | ND | ND | 18.2H | 31 | 10.2 | ND | ND | ND | ND | ND |
| 22 | ND | ND | ND | 392 | ND | ND | 18.9H | 34.4 | 11.4 | ND | ND | ND | ND | ND |
| 31 | ND | ND | ND | 408 | ND | ND | 15.4 | 36.1 | 12.1 | ND | ND | ND | ND | ND |
| 42 | ND | ND | ND | 399 | ND | ND | 16.4 | 41.2 | 13.3 | ND | ND | ND | ND | ND |
| 49 | ND | ND | ND | 398 | ND | ND | 16.4 | 43 | 14.2 | ND | ND | ND | ND | ND |
| 56 | ND | ND | ND | 362 | ND | ND | 16.5 | 40.5 | 13.5 | ND | ND | ND | ND | ND |
| 63 | ND | ND | ND | 266 | ND | ND | 16.4 | 43.9 | 14.3 | ND | ND | ND | ND | ND |
| 121 | 156 | 0.1 | < 5 | 303 | ND | ND | 11.6 | 52.9 | 18 | 159 | 41 | 1 | 5.5 | 3.4 |
Hemophilia B dog G11 received 8.57 × 1011 vps/kg body weight HD vector AdFTC/cFIX/ChMAR. Samples were collected periodically and the samples on day 0 and before were obtained prior to any treatment.
Alk phos indicates alkaline phosphatase; GGTP, γ-glytamyltransferase; PLT, platelets; % FIX activity % NCP, percent cFIX activity of pooled normal canine plasma; WBC, white blood cell; HCT, hematocrit; HGB, hemoglobin; CPK, creatine phosphokinase; Creat, creatinine; and ND, not determined.
Most significant measurements.