Purity and titer of the final adenoviral vector preparations
Viral preparation . | Total particle yield . | Total no. of transducing units . | Helper virus contamination, % . |
|---|---|---|---|
| 1 | 7.8 × 1011 | 3.9 × 1010 | 0.0031 |
| 2 | 3.0 × 1011 | 1.5 × 1010 | 0.0036 |
| 3 | 3.0 × 1010 | 1.5 × 109 | 0.02 |
| 4 | 5.4 × 1011 | 2.7 × 1010 | 0.009 |
Viral preparation . | Total particle yield . | Total no. of transducing units . | Helper virus contamination, % . |
|---|---|---|---|
| 1 | 7.8 × 1011 | 3.9 × 1010 | 0.0031 |
| 2 | 3.0 × 1011 | 1.5 × 1010 | 0.0036 |
| 3 | 3.0 × 1010 | 1.5 × 109 | 0.02 |
| 4 | 5.4 × 1011 | 2.7 × 1010 | 0.009 |
The table summarizes contamination levels with the helper virus used for vector production. Shown are 4 representative preparations of the HD vector AdFTC/cFIX/ChMAR, which were used for hepatic infusion into hemophilia B dogs. The level of helper virus contamination was defined as the percentage of transducing helper virus particles contained in the total amount of transducing HD vector units.