VH and sequence analysis of IgG aCL (GVH and GVL) originating from single cell sorting (patient 1)
. | Vhfamily . | Vkfamily . | Germline gene . | Homology (%)* . | R/S CDR . | R/S FR . | D gene . | Jhgene . | Jkgene . |
|---|---|---|---|---|---|---|---|---|---|
| GVH1 | 1 | — | V1-69 | 95.6 | 3/0 | 3/5 | D1-20/in† | 4 | — |
| P = .09‡ | |||||||||
| GVH2 | 1 | — | V1-69 | 99.2 | — | 0/2 | D3-16 | 3 | — |
| GVH3 | 2 | — | V2-5 | 100 | — | — | D3-3 | 5 | — |
| GVH4 | 3 | — | V3-21 | 97.6 | 1/0 | 4/1 | D5-5 | 4 | — |
| P = .339 | |||||||||
| GVH5 | 3 | — | V3-23 | 93.9 | 7/5 | ½ | D1-14 | 3 | — |
| P = .0027 | |||||||||
| GVH6 | 3 | — | V3-30 | 100 | — | ND | 3 | — | |
| GVH7 | 4 | — | V4-4 | 92.2 | 5/3 | 6/5 | D4-41 | 4 | — |
| GVH8 | 3 | — | V3-7 | 95.5 | 5/1 | 4/1 | D6-13 | 4 | — |
| P = .015 | |||||||||
| GVH9 | 3 | — | V3-73 | 92.5 | 5/3 | 6/5 | D3-22 | 6 | — |
| GVL5 | 1 | V1-9 | 98.8 | 2/0 | 1/0 | — | 3 | ||
| GVL6 | — | 3 | V3-11 | 96.7 | 4/0 | 2/1 | — | 2 | |
| P = .0025 | |||||||||
| GVL7 | — | 1 | V1-9 | 99.7 | — | 1/0 | — | — | 3 |
| GVL8 | — | 1 | V1-33 | 99.6 | — | 1/0 | — | — | 1 |
| GVL9 | — | 1 | V1-39 | 97.2 | 3/1 | 2/1 | — | — | 2 |
| P = .013 |
. | Vhfamily . | Vkfamily . | Germline gene . | Homology (%)* . | R/S CDR . | R/S FR . | D gene . | Jhgene . | Jkgene . |
|---|---|---|---|---|---|---|---|---|---|
| GVH1 | 1 | — | V1-69 | 95.6 | 3/0 | 3/5 | D1-20/in† | 4 | — |
| P = .09‡ | |||||||||
| GVH2 | 1 | — | V1-69 | 99.2 | — | 0/2 | D3-16 | 3 | — |
| GVH3 | 2 | — | V2-5 | 100 | — | — | D3-3 | 5 | — |
| GVH4 | 3 | — | V3-21 | 97.6 | 1/0 | 4/1 | D5-5 | 4 | — |
| P = .339 | |||||||||
| GVH5 | 3 | — | V3-23 | 93.9 | 7/5 | ½ | D1-14 | 3 | — |
| P = .0027 | |||||||||
| GVH6 | 3 | — | V3-30 | 100 | — | ND | 3 | — | |
| GVH7 | 4 | — | V4-4 | 92.2 | 5/3 | 6/5 | D4-41 | 4 | — |
| GVH8 | 3 | — | V3-7 | 95.5 | 5/1 | 4/1 | D6-13 | 4 | — |
| P = .015 | |||||||||
| GVH9 | 3 | — | V3-73 | 92.5 | 5/3 | 6/5 | D3-22 | 6 | — |
| GVL5 | 1 | V1-9 | 98.8 | 2/0 | 1/0 | — | 3 | ||
| GVL6 | — | 3 | V3-11 | 96.7 | 4/0 | 2/1 | — | 2 | |
| P = .0025 | |||||||||
| GVL7 | — | 1 | V1-9 | 99.7 | — | 1/0 | — | — | 3 |
| GVL8 | — | 1 | V1-33 | 99.6 | — | 1/0 | — | — | 1 |
| GVL9 | — | 1 | V1-39 | 97.2 | 3/1 | 2/1 | — | — | 2 |
| P = .013 |
R/S indicates the ratio of replacement mutations (R) and silent mutations (S) in the complementary-determining regions (CDR1 and CDR2) and framework (FR1, FR2, and FR3); and—, not applicable.
Homology % provides the percentage of nucleotide sequence homology between the Ig V gene and its putative germ line.
In indicates sequence inserted in inverted orientation.
P is the probability that an excess of R mutations in the CDRs resulted by chance.