Differential gene induction by TLR agonists in neutrophils
TLR agonist inducible . | Constitutively expressed . | No/low expression . |
|---|---|---|
| IL-8 | IFN-α, -β, -γ | IL-4 |
| MIP-1α | TNF | IL-6 |
| MIP-1β | β-defensins-1, -2, -3 | IL-10 |
| MIP-3α | α-defensin-1 | MIG |
| GRO-α | MCP-1 | IP-10 |
| IL-1β | IL-12 p40 | I-TAC |
| RANTES | ||
| MIP-3β | ||
| BCA-1 |
TLR agonist inducible . | Constitutively expressed . | No/low expression . |
|---|---|---|
| IL-8 | IFN-α, -β, -γ | IL-4 |
| MIP-1α | TNF | IL-6 |
| MIP-1β | β-defensins-1, -2, -3 | IL-10 |
| MIP-3α | α-defensin-1 | MIG |
| GRO-α | MCP-1 | IP-10 |
| IL-1β | IL-12 p40 | I-TAC |
| RANTES | ||
| MIP-3β | ||
| BCA-1 |
Total RNA was isolated from primary human neutrophils (1 × 106 cells) stimulated with LPS (100 ng/mL), lipopeptide (100 ng/mL), poly I:C (50 μg/mL), flagellin (300 ng/mL), CpG DNA (50 μg/mL), zymosan (50 μg/mL), R848 (10 μM), and GM-CSF (50 ng/mL) for 1 hour. Expression of chemokines and cytokines was quantified by QPCR. Table lists genes induced after TLR stimulation, genes expressed constitutively with no induction after TLR stimulation, and genes not expressed or expressed at very low levels with no induction after TLR stimulation.