Primers used for QPCR
Human mRNA . | Forward primer . | Reverse primer . | Accession no. . |
|---|---|---|---|
| CXCL1/GRO-α | ACGTGAAGTCCCCCGGAC | GCCCATTCTTGAGTGTGGCT | NM_001511 |
| CXCL8/IL-8 | CTGGCCGTGGCTCTCTTG | CCTTGGCAAAACTGCACCTT | NM_000584 |
| CXCL9/MIG | TGCAAGGAACCCCAGTAGTGA | GGTGGATAGTCCCTTGGTTGG | NM_002416 |
| CXCL10/IP-10 | TGAAATTATTCCTGCAAGCCAA | CAGACATCTCTTCTCACCCTTCTTT | NM_001565 |
| CXCL11/I-TAC | CAAGGCTTCCCCATGTTCA | CCACTTTCACTGCTTTTACCCC | NM_005409 |
| CXCL13/BCA | GACGCTTCATTGATCGAATTCA | TTCCAGACTATGATTTCTTTTCTTGG | NM_006419 |
| CCL2/MCP-1 | CTCTGCCGCCCTTCTGTG | TGCATCTGGCTGAGCGAG | NM_002982 |
| CCL3/MIP-1α | AGCTGACTACTTTGAGACGAGCA | CGGCTTCGCTTGGTTAGGA | NM_002983 |
| CCL4/MIP-1β | CTGCTCTCCAGCGCTCTCA | GTAAGAAAAGCAGCAGGCGG | NM_002984 |
| CCL5/RANTES | GACACCACACCCTGCTGCT | TACTCCTTGATGTGGGCACG | NM_002985 |
| CCL19/MIP-3β | GGCACCAATGATGCTGAAGA | GAAGTTCCTCACGATGTACCCAG | NM_006274 |
| CCL20/MIP-3α | TCCTGGCTGCTTTGATGTCA | TCAAAGTTGCTTGCTGCTTCTG | NM_004591 |
| IL-1β | ACGAATCTCCGACCACCACT | CCATGGCCACAACAACTGAC | M15330 |
| IL-4 | GGCAGTTCTACAGCCACCATG | GCCTGTGGAACTGCTGTGC | M23442 |
| IL-6 | GACCCAACCACAAATGCCA | GTCATGTCCTGCAGCCACTG | M14584 |
| IL-10 | GGTGATGCCCCAAGCTGA | TCCCCCAGGGAGTTCACA | U16720 |
| IL-12 p40 | CGGTCATCTGCCGCAAA | CAAGATGAGCTATAGTAGCGGTCCT | M65272 |
| IFN-α | GGTGCTCAGCTGCAAGTCAA | GCTACCCAGGCTGTGGGTT | J00207 |
| IFN-β | CAGCAATTTTCAGTGTCAGAAGC | TCATCCTGTCCTTGAGGCAGT | M28622 |
| IFN-γ | CCAACGCAAAGCAATACATGA | CGCTTCCCTGTTTTAGCTGC | J00219 |
| TNF | GGTGCTTGTTCCTCAGCCTC | CAGGCAGAAGAGCGTGGTG | M10988 |
| β-defensin-1 | GCAGTGGAGGGCAATGTCTC | TTCCCTCTGTAACAGGTGCCTT | NM_005218 |
| β-defensin-2 | TGATGCCTCTTCCAGGTGTTT | GGATGACATATGGCTCCACTCTTA | AF040153 |
| β-defensin-3 | TTATTGCAGAGTCAGAGGCGG | CGAGCACTTGCCGATCTGTT | AF516673 |
| α-defensin-1 | TCCCAGAAGTGGTTGTTTCCC | TCCTTGAGCCTGGATGCTTT | NM_004084 |
| TLR1 | CTGGTATCTCAGGATGGTGTGC | TTGGAGTTCTTCTAAGGGTATGTTCC | NM_003263 |
| TLR2 | GGCCAGCAAATTACCTGTGTG | AGGCGGACATCCTGAACCT | NM_003264 |
| TLR3 | TCCCAAGCCTTCAACGACTG | TGGTGAAGGAGAGCTATCCACA | NM_003265 |
| TLR4 | CTGCAATGGATCAAGGACCA | TTATCTGAAGGTGTTGCACATTCC | XM_057452 |
| TLR5 | TCGAGCCCCTACAAGGGAA | CACTGAGACTCTGCTATACAAGCTA | NM_003268 |
| TLR6 | CTATTGTTAAAAGCTTCCATTTTGT | ACCTGAAGCTCAGCGATGTAGTTC | NM_006068 |
| TLR7 | TTACCTGGATGGAAACCAGCTAC | TCAAGGCTGAGAAGCTGTAAGCTA | NM_016562 |
| TLR8 | GAGAGCCGAGACAAAAACGTTC | TGTCGATGATGGCCAATCC | NM_016610 |
| TLR9 | TGGTGTTGAAGGACAGTTCTCTC | CACTCGGAGGTTTCCCAGC | NM_017442 |
| TLR10 | GAAAGGTTCCCGCAGACTTG | TGGAGTTGAAAAAGGAGGTTATAG | NM_030956 |
| β2-microglobulin | CTCCGTGGCCTTAGCTGTG | TTTGGAGTACGCTGGATAGCCT | AF072097 |
Human mRNA . | Forward primer . | Reverse primer . | Accession no. . |
|---|---|---|---|
| CXCL1/GRO-α | ACGTGAAGTCCCCCGGAC | GCCCATTCTTGAGTGTGGCT | NM_001511 |
| CXCL8/IL-8 | CTGGCCGTGGCTCTCTTG | CCTTGGCAAAACTGCACCTT | NM_000584 |
| CXCL9/MIG | TGCAAGGAACCCCAGTAGTGA | GGTGGATAGTCCCTTGGTTGG | NM_002416 |
| CXCL10/IP-10 | TGAAATTATTCCTGCAAGCCAA | CAGACATCTCTTCTCACCCTTCTTT | NM_001565 |
| CXCL11/I-TAC | CAAGGCTTCCCCATGTTCA | CCACTTTCACTGCTTTTACCCC | NM_005409 |
| CXCL13/BCA | GACGCTTCATTGATCGAATTCA | TTCCAGACTATGATTTCTTTTCTTGG | NM_006419 |
| CCL2/MCP-1 | CTCTGCCGCCCTTCTGTG | TGCATCTGGCTGAGCGAG | NM_002982 |
| CCL3/MIP-1α | AGCTGACTACTTTGAGACGAGCA | CGGCTTCGCTTGGTTAGGA | NM_002983 |
| CCL4/MIP-1β | CTGCTCTCCAGCGCTCTCA | GTAAGAAAAGCAGCAGGCGG | NM_002984 |
| CCL5/RANTES | GACACCACACCCTGCTGCT | TACTCCTTGATGTGGGCACG | NM_002985 |
| CCL19/MIP-3β | GGCACCAATGATGCTGAAGA | GAAGTTCCTCACGATGTACCCAG | NM_006274 |
| CCL20/MIP-3α | TCCTGGCTGCTTTGATGTCA | TCAAAGTTGCTTGCTGCTTCTG | NM_004591 |
| IL-1β | ACGAATCTCCGACCACCACT | CCATGGCCACAACAACTGAC | M15330 |
| IL-4 | GGCAGTTCTACAGCCACCATG | GCCTGTGGAACTGCTGTGC | M23442 |
| IL-6 | GACCCAACCACAAATGCCA | GTCATGTCCTGCAGCCACTG | M14584 |
| IL-10 | GGTGATGCCCCAAGCTGA | TCCCCCAGGGAGTTCACA | U16720 |
| IL-12 p40 | CGGTCATCTGCCGCAAA | CAAGATGAGCTATAGTAGCGGTCCT | M65272 |
| IFN-α | GGTGCTCAGCTGCAAGTCAA | GCTACCCAGGCTGTGGGTT | J00207 |
| IFN-β | CAGCAATTTTCAGTGTCAGAAGC | TCATCCTGTCCTTGAGGCAGT | M28622 |
| IFN-γ | CCAACGCAAAGCAATACATGA | CGCTTCCCTGTTTTAGCTGC | J00219 |
| TNF | GGTGCTTGTTCCTCAGCCTC | CAGGCAGAAGAGCGTGGTG | M10988 |
| β-defensin-1 | GCAGTGGAGGGCAATGTCTC | TTCCCTCTGTAACAGGTGCCTT | NM_005218 |
| β-defensin-2 | TGATGCCTCTTCCAGGTGTTT | GGATGACATATGGCTCCACTCTTA | AF040153 |
| β-defensin-3 | TTATTGCAGAGTCAGAGGCGG | CGAGCACTTGCCGATCTGTT | AF516673 |
| α-defensin-1 | TCCCAGAAGTGGTTGTTTCCC | TCCTTGAGCCTGGATGCTTT | NM_004084 |
| TLR1 | CTGGTATCTCAGGATGGTGTGC | TTGGAGTTCTTCTAAGGGTATGTTCC | NM_003263 |
| TLR2 | GGCCAGCAAATTACCTGTGTG | AGGCGGACATCCTGAACCT | NM_003264 |
| TLR3 | TCCCAAGCCTTCAACGACTG | TGGTGAAGGAGAGCTATCCACA | NM_003265 |
| TLR4 | CTGCAATGGATCAAGGACCA | TTATCTGAAGGTGTTGCACATTCC | XM_057452 |
| TLR5 | TCGAGCCCCTACAAGGGAA | CACTGAGACTCTGCTATACAAGCTA | NM_003268 |
| TLR6 | CTATTGTTAAAAGCTTCCATTTTGT | ACCTGAAGCTCAGCGATGTAGTTC | NM_006068 |
| TLR7 | TTACCTGGATGGAAACCAGCTAC | TCAAGGCTGAGAAGCTGTAAGCTA | NM_016562 |
| TLR8 | GAGAGCCGAGACAAAAACGTTC | TGTCGATGATGGCCAATCC | NM_016610 |
| TLR9 | TGGTGTTGAAGGACAGTTCTCTC | CACTCGGAGGTTTCCCAGC | NM_017442 |
| TLR10 | GAAAGGTTCCCGCAGACTTG | TGGAGTTGAAAAAGGAGGTTATAG | NM_030956 |
| β2-microglobulin | CTCCGTGGCCTTAGCTGTG | TTTGGAGTACGCTGGATAGCCT | AF072097 |