Recipients of SHIP−/− bone marrow cells have a lower frequency of donor-derived cells and repopulation is skewed toward the myeloid lineage
Transplant source and cell dose . | % Ly5.2+ peripheral blood . | Myeloid-B-lymphoid ratio in donor-derived Ly5.2+ cells . |
|---|---|---|
| 2.5 × 105 | ||
| SHIP+/+ | 88.4 ± 4.3 | 0.22 ± 0.12 |
| SHIP−/− | 61.3 ± 8.7* | 1.87 ± 2.02 |
| 5.0 × 105 | ||
| SHIP+/+ | 93.5 ± 1.6 | 0.15 ± 0.06 |
| SHIP−/− | 62.0 ± 13.3* | 1.56 ± 1.37† |
| 1.0 × 106 | ||
| SHIP+/+ | 93.5 ± 1.1 | 0.19 ± 0.04 |
| SHIP−/− | 73.6 ± 6.8* | 1.70 ± 1.37† |
Transplant source and cell dose . | % Ly5.2+ peripheral blood . | Myeloid-B-lymphoid ratio in donor-derived Ly5.2+ cells . |
|---|---|---|
| 2.5 × 105 | ||
| SHIP+/+ | 88.4 ± 4.3 | 0.22 ± 0.12 |
| SHIP−/− | 61.3 ± 8.7* | 1.87 ± 2.02 |
| 5.0 × 105 | ||
| SHIP+/+ | 93.5 ± 1.6 | 0.15 ± 0.06 |
| SHIP−/− | 62.0 ± 13.3* | 1.56 ± 1.37† |
| 1.0 × 106 | ||
| SHIP+/+ | 93.5 ± 1.1 | 0.19 ± 0.04 |
| SHIP−/− | 73.6 ± 6.8* | 1.70 ± 1.37† |
Irradiated Pep3b (Ly5.1+) recipients were injected with varying repopulating doses (2.5 to 10 × 105 cells per mouse) of SHIP+/+ or SHIP−/− bone marrow cells (4 independent experiments; 4-8 recipients per cell dose). Donor-derived contributions were evaluated by monitoring the percentage of Ly5.2+, B220+, and/or Mac1+ cells in the periphery at 16 weeks after transplantation.
P < .001.
P < .005 versus SHIP+/+ at the same cell dose.