Summary of experimental parameters used to determine the frequency of CRU in primary recipients of SHIP+/+and SHIP−/−BM
Cell dose injected . | Number positive . | Number analyzed . | |
|---|---|---|---|
| SHIP+/+ | |||
| 10 000 | 0 | 4 | |
| 50 000 | 0 | 3 | |
| 100 000 | 3 | 4 | |
| 250 000 | 2 | 3 | |
| 500 000 | 6 | 6 | |
| 1 000 000 | 8 | 8 | |
| 5 000 000 | 2 | 2 | |
| 10 000 000 | 5 | 5 | |
| CRU frequency | 1/148 102 (95% CI: 1/73 400-1/298 900) | ||
| SHIP−/− | |||
| 3240 | 0 | 6 | |
| 50 000 | 0 | 3 | |
| 10 0000 | 0 | 4 | |
| 160 000 | 0 | 6 | |
| 500 000 | 1 | 5 | |
| 800 000 | 2 | 3 | |
| 1 000 000 | 2 | 5 | |
| 5 000 000 | 4 | 5 | |
| CRU frequency | 1/2 346 773 (95% CI: 1/1 135 500-1/4 850 070)* | ||
Cell dose injected . | Number positive . | Number analyzed . | |
|---|---|---|---|
| SHIP+/+ | |||
| 10 000 | 0 | 4 | |
| 50 000 | 0 | 3 | |
| 100 000 | 3 | 4 | |
| 250 000 | 2 | 3 | |
| 500 000 | 6 | 6 | |
| 1 000 000 | 8 | 8 | |
| 5 000 000 | 2 | 2 | |
| 10 000 000 | 5 | 5 | |
| CRU frequency | 1/148 102 (95% CI: 1/73 400-1/298 900) | ||
| SHIP−/− | |||
| 3240 | 0 | 6 | |
| 50 000 | 0 | 3 | |
| 10 0000 | 0 | 4 | |
| 160 000 | 0 | 6 | |
| 500 000 | 1 | 5 | |
| 800 000 | 2 | 3 | |
| 1 000 000 | 2 | 5 | |
| 5 000 000 | 4 | 5 | |
| CRU frequency | 1/2 346 773 (95% CI: 1/1 135 500-1/4 850 070)* | ||
To determine in vivo CRU regeneration levels, groups of recipients were killed 16 to 20 weeks after transplantation of 106 BM cells from SHIP+/+ or SHIP−/− mice, and the BM from these mice was evaluated by transplantation of the indicated cell doses into secondary Pep3b mice in a CRU assay. Mice reconstituted to 1% or more donor-type myeloid and 1% or more donor-type B-lymphoid cells were scored as positive.
P <.0001 versus wild type.