Effect of anti-C5 and anti-C5a on serum bactericidal activity
Bactericidal activity . | No antibody . | Isotype control . | Anti-C5a 137-26 . | Anti-C5 137-76 . |
|---|---|---|---|---|
| L3, 7, 9 (positive control) | 14 | 15 | 15 | < 8 |
| L8 (negative control) | < 8 | < 8 | < 8 | < 8 |
| Subject 1 | 6 | 5 | 5 | < 1 |
| Subject 2 | 7 | 8 | 7 | < 1 |
Bactericidal activity . | No antibody . | Isotype control . | Anti-C5a 137-26 . | Anti-C5 137-76 . |
|---|---|---|---|---|
| L3, 7, 9 (positive control) | 14 | 15 | 15 | < 8 |
| L8 (negative control) | < 8 | < 8 | < 8 | < 8 |
| Subject 1 | 6 | 5 | 5 | < 1 |
| Subject 2 | 7 | 8 | 7 | < 1 |
Bactericidal activity (agar overlay method) of serum of the 2 control subjects and of anti-LPS L3,7,9 lgG2a antibody (positive control) and anti-LPS L8 lgG1 antibody (negative control) expressed as log2 titer of the last reciprocal serum dilution at which more than 50% of the inoculum was killed. Both control subjects showed excellent serum bactericidal activity against N meningitidis H44/76-1. Anti-C5 completely inhibited all bactericidal activity, whereas anti-C5a and isotype control had no effect.