Table 4. Nucleotide and amino acid... | American Society of Hematology

Table 4.

Nucleotide and amino acid sequence of the TCR α chain of HA-2-specific T-cell clones

Clone group	v alpha	N region	J alpha	C alpha
A	AV5S1		42
	TGT GCA GAG	ACT GCG GGT	TAT GGA GGA AGC CAA GGA AAT CTC CAT CTT TGG AAA AGG CAC TAA ACT CTC TGT TAA ACC	AAT ATC
	C A E	T A G	Y G G S Q G N L I F G K G T K L S V K P	N I
B	AV26S1		42
	ACT CGC ATC	CTG AGA CCT GGG ATG AAT	TAT GGA GGA AGC CAA GGA AAT CTC CAT CTT TGG AAA AGG CAC TAA ACT CTC TGT TAA ACC	AAT ATC
	Y C I	L R P G M N	Y G G S Q G N L I F G K G T K L S V K P	N I
C	AV21S1		42
	TGT GCT GTG	AGG CCG GGG AAT	TAT GGA GGA AGC CAA GGA AAT CTC CAT CTT TGG AAA AGG CAC TAA ACT CTC TGT TAA ACC	AAT ATC
	C A V	R P G N	Y G G S Q G N L I F G K G T K L S V K P	N I
D	AV20S1		42
	TGT GCT GTG	CAG GCA GGC	TAT GGA GGA AGC CAA GGA AAT CTC CAT CTT TGG AAA AGG CAC TAA ACT CTC TGT TAA ACC	AAT ATC
	C A V	Q A G	Y G G S Q G N L I F G K G T K L S V K P	N I
E	ADV38S2		30
	CTA GTG CTT	ATA GGG GAC	AGA GAT GAC AAG ATC ATC TTT GGA AAA GGG ACA CGA CTT CAT ATT CTC CCC	AAT ATC
	L V L	I G D	R D D K I I F G K G T R L H I L P	N I
	AV21S1		42
	TGT GCT GTG	AGG CCT AAG AAT	TAT GGA GGA AGC CAA GGA AAT CTC CAT CTT TGG AAA AGG CAC TAA ACT CTC TGT TAA ACC	AAT ATC
	C A V	R P K N	Y G G S Q G N L T F G K G T K L S V K P	N I
F	AV21S1		42
	TGT GCT GTG	AGG TCA GGG	TAT GGA GGA AGC CAA GGA AAT CTC CAT CTT TGG AAA AGG CAC TAA ACT CTC TGT TAA ACC	AAT ATC
	C A V	R S G	Y G G S Q G N L I F G K G T K L S V K P	N I
G	AV21S1		42
	TGT GCT GTG	AGG CCA GGA AAT	TAT GGA GGA AGC CAA GGA AAT CTC CAT CTT TGG AAA AGG CAC TAA ACT CTC TGT TAA ACC	AAT ATC
	C A V	R P G N	Y G G S Q G N L I F G K G T K L S V K P	N I
H	AV21S1		42
	TGT GCT GTG	AGA TAT AAG AAT	TAT GGA GGA AGC CAA GGA AAT CTC CAT CTT TGG AAA AGG CAC TAA ACT CTC TGT TAA ACC	AAT ATC
	C A V	R Y K N	Y G G S Q G N L I F G K G T K L S V K P	N I
I	AV35S1		42
	TTC TGT GCT	GGG CCC ATG CCG ACT	TAT GGA GGA AGC CAA GGA AAT CTC CAT CTT TGG AAA AGG CAC TAA ACT CTC TGT TAA ACC	AAT ATC
	F C A	G P M P T	Y G G S Q G N L I F G K G T K L S V K P	N I
	AV25S1		34
	CTG TGC AGG	ATG AGC TAT GGC	ACC GAC AAG CTC ATC TTT GGG ACT GGG ACC AGA TTA CAA GTC TTT	AAT ATC
	N L L	L C R G	T D K L I F G T G T R L Q V F	N I
J	AV20S1		42
	TGT GCT GTG	CAG GCC GGT	TAT GGA GGA AGC CAA GGA AAT CTC CAT CTT TGG AAA AGG CAC TAA ACT CTC TGT TAA ACC	AAT ATC
	C A V	Q A G	Y G G S Q G N L I F G K G T K L S V K P	N I
K	AV21S1		42
	TGT GCT GTG	AGG CCG ATG AAT	TAT GGA GGA AGC CAA GGA AAT CTC CAT CTT TGG AAA AGG CAC TAA ACT CTC TGT TAA ACC	AAT ATC
	C A V	R P M N	Y G G S Q G N L I F G K G T K L S V K P	N I
L	AV21S1		42
	TGT GCT GTG	AGG CGG GGC	TAT GGA GGA AGC CAA GGA AAT CTC CAT CTT TGG AAA AGG CAC TAA ACT CTC TGT TAA ACC	AAT ATC
	C A V	R R G	Y G G S Q G N L I F G R G T K L S V K P	N I
5H13	AV35S1		42
	TTC TGT AGG	GGC CCC CCT CGT AAT	TAT GGA GGA AGC CAA GGA AAT CTC CAT CTT TGG AAA AGG CAC TAA ACT CTC TGT TAA ACC	AAT ATC
	F C A	G P P R N	Y G G S Q G N L I F G K G T K L S V K P	N I

Clone group	v alpha	N region	J alpha	C alpha
A	AV5S1		42
	TGT GCA GAG	ACT GCG GGT	TAT GGA GGA AGC CAA GGA AAT CTC CAT CTT TGG AAA AGG CAC TAA ACT CTC TGT TAA ACC	AAT ATC
	C A E	T A G	Y G G S Q G N L I F G K G T K L S V K P	N I
B	AV26S1		42
	ACT CGC ATC	CTG AGA CCT GGG ATG AAT	TAT GGA GGA AGC CAA GGA AAT CTC CAT CTT TGG AAA AGG CAC TAA ACT CTC TGT TAA ACC	AAT ATC
	Y C I	L R P G M N	Y G G S Q G N L I F G K G T K L S V K P	N I
C	AV21S1		42
	TGT GCT GTG	AGG CCG GGG AAT	TAT GGA GGA AGC CAA GGA AAT CTC CAT CTT TGG AAA AGG CAC TAA ACT CTC TGT TAA ACC	AAT ATC
	C A V	R P G N	Y G G S Q G N L I F G K G T K L S V K P	N I
D	AV20S1		42
	TGT GCT GTG	CAG GCA GGC	TAT GGA GGA AGC CAA GGA AAT CTC CAT CTT TGG AAA AGG CAC TAA ACT CTC TGT TAA ACC	AAT ATC
	C A V	Q A G	Y G G S Q G N L I F G K G T K L S V K P	N I
E	ADV38S2		30
	CTA GTG CTT	ATA GGG GAC	AGA GAT GAC AAG ATC ATC TTT GGA AAA GGG ACA CGA CTT CAT ATT CTC CCC	AAT ATC
	L V L	I G D	R D D K I I F G K G T R L H I L P	N I
	AV21S1		42
	TGT GCT GTG	AGG CCT AAG AAT	TAT GGA GGA AGC CAA GGA AAT CTC CAT CTT TGG AAA AGG CAC TAA ACT CTC TGT TAA ACC	AAT ATC
	C A V	R P K N	Y G G S Q G N L T F G K G T K L S V K P	N I
F	AV21S1		42
	TGT GCT GTG	AGG TCA GGG	TAT GGA GGA AGC CAA GGA AAT CTC CAT CTT TGG AAA AGG CAC TAA ACT CTC TGT TAA ACC	AAT ATC
	C A V	R S G	Y G G S Q G N L I F G K G T K L S V K P	N I
G	AV21S1		42
	TGT GCT GTG	AGG CCA GGA AAT	TAT GGA GGA AGC CAA GGA AAT CTC CAT CTT TGG AAA AGG CAC TAA ACT CTC TGT TAA ACC	AAT ATC
	C A V	R P G N	Y G G S Q G N L I F G K G T K L S V K P	N I
H	AV21S1		42
	TGT GCT GTG	AGA TAT AAG AAT	TAT GGA GGA AGC CAA GGA AAT CTC CAT CTT TGG AAA AGG CAC TAA ACT CTC TGT TAA ACC	AAT ATC
	C A V	R Y K N	Y G G S Q G N L I F G K G T K L S V K P	N I
I	AV35S1		42
	TTC TGT GCT	GGG CCC ATG CCG ACT	TAT GGA GGA AGC CAA GGA AAT CTC CAT CTT TGG AAA AGG CAC TAA ACT CTC TGT TAA ACC	AAT ATC
	F C A	G P M P T	Y G G S Q G N L I F G K G T K L S V K P	N I
	AV25S1		34
	CTG TGC AGG	ATG AGC TAT GGC	ACC GAC AAG CTC ATC TTT GGG ACT GGG ACC AGA TTA CAA GTC TTT	AAT ATC
	N L L	L C R G	T D K L I F G T G T R L Q V F	N I
J	AV20S1		42
	TGT GCT GTG	CAG GCC GGT	TAT GGA GGA AGC CAA GGA AAT CTC CAT CTT TGG AAA AGG CAC TAA ACT CTC TGT TAA ACC	AAT ATC
	C A V	Q A G	Y G G S Q G N L I F G K G T K L S V K P	N I
K	AV21S1		42
	TGT GCT GTG	AGG CCG ATG AAT	TAT GGA GGA AGC CAA GGA AAT CTC CAT CTT TGG AAA AGG CAC TAA ACT CTC TGT TAA ACC	AAT ATC
	C A V	R P M N	Y G G S Q G N L I F G K G T K L S V K P	N I
L	AV21S1		42
	TGT GCT GTG	AGG CGG GGC	TAT GGA GGA AGC CAA GGA AAT CTC CAT CTT TGG AAA AGG CAC TAA ACT CTC TGT TAA ACC	AAT ATC
	C A V	R R G	Y G G S Q G N L I F G R G T K L S V K P	N I
5H13	AV35S1		42
	TTC TGT AGG	GGC CCC CCT CGT AAT	TAT GGA GGA AGC CAA GGA AAT CTC CAT CTT TGG AAA AGG CAC TAA ACT CTC TGT TAA ACC	AAT ATC
	F C A	G P P R N	Y G G S Q G N L I F G K G T K L S V K P	N I

Only the last 3 residues of the V region are shown followed by the N region, the J region, and the first two residues of the C region. T-cell clone 5H13, also specific for the mHag HA-2, was derived from an unrelated donor.

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