Table 3. Nucleotide and amino acid... | American Society of Hematology

Table 3.

Nucleotide and amino acid sequence of the TCR β chain of HA-2-specific T-cell clones

Clone group	V beta	N-D-N region	J beta	C beta
A	BV18S1		2S1	2
	TGT GCC AGC	TCC CGG ACT AGC GGG GCT AAC	AAT GAG CAG TTC TTC GGG CCA GGG ACA CGG CTC ACC GTG CTA	GAG GAC
	C A S	S R T S G A N	N E Q F F G P G T R L T V L	E D
B	BV7S8		2S7	2
	TGT GCC AGC	AGC GCA GTA GCG GGG ACC	TCC TAC GAG CAG TAC TTC GGG CCG GGC ACC AGG CTC ACG GTC ACA	GAG GAC
	C A S	S A V A G T	S Y E Q Y F G P G T R L T V T	E D
C	BV7S8		1S4	1
	TGT GCC AGC	AGC TTA GGC CTT TGG GGG AC GAT AAT	GAA AAA CTG TTT TTT GGC AGT GGA ACC CAG CTC TCT GTC TTG	GAG GAC
	C A S	S L G L W G T D N	E K L F F G S G T Q L S V L	E D
D	BV18S1		2S2	2
	TGT GCC AGC	TCC CCG CCC AGC GGG GGT TCG	ACC GGG GAG CTG TTT TTT GGA GAA GGC TCT AGG CTG ACC GTA CTG	GAG GAC
	C A S	S P P S G G S	T G E L F F G E G S R L T V L	E D
E	BV7S8		2S2	2
	TGT GCC AGC	AGA AAA GCG GGA CAG GAA AAC	ACC GGG GAG CTG TTT TTT GGA GAA GGC TCT AGG CTG ACC GTA CTG	GAG GAC
	C A S	R K A G Q E N	T G E L F F G E G S R L T V L	E D
F	BV7S8		2S3	2
	TGT GCC AGC	TTA TTT CCC GGT CAG GAC AGG GGA CAC GGG ACT AGC GGC AGC ACA GAT	ACG CAG TAT TTT GGC CCA GGC ACC CGG CTG ACA GTG CTC	GAG GAC
	C A S	L F P G Q D R G H G T S G S T D	T Q Y F G P G T R L T V L	E D
G	BV7S8		2S1	2
	TGT GCC AGC	AGC TTA GTA CCG	AAT GAG CAG TTC TTC GGG CCA GGG ACA CGG CTC ACC GTG CTA	GAG GAC
	C A S	S L V P	N E Q F F G P G T R L T V L	E D
H	BV18S1		2S1	2
	TGT GCC AGC	TCA CCA CTG GGC TCC TAC	AAT GAG CAG TTC TTC GGG CCA GGG ACA CGG CTC ACC GTG CTA	GAG GAC
	C A S	S P L G S Y	N E Q F F G P G T R L T V L	E D
I	BV7S8		2S3	2
	TGT GCC AGC	AGC CCC CCT AGC GGG AGG TAT	ACG CAG TAT TTT GGC CCA GGC ACC CGG CTG ACA GTG CTC	GAG GAC
	C A S	S P P S G R Y	T Q Y F G P G T R L T V L	E D
	BV10S3		1S1	1
	TGT GCC ATC	AGT GAG TCG CGA ACA GGG ATC GCT	GAA GCT TTC TTT GGA CAA GGC ACC AGA CTC ACA GTT GTA	GAG GAC
	C A I	S E S R T G I A	E A F F G Q G T R L T V V	E D
J	BV18S1		1S6	1
	TGT GCC AGC	TCA CCG ACA GGG GGG TCG CGA	TCA CCC CTC CAC TTT GGG AAC GGG ACC AGG CTC ACT GTG ACA	GAG GAC
	C A S	S P T G G S R	S P L H F G N G T R L T V T	E D
K	BV7S8		2S1	2
	TGT GCC AGC	AGC TGG CCG GAC ACC TAC	AAT GAG CAG TTC TTC GGG CCA GGG ACA CGG CTC ACC GTG CTA	GAG GAC
	C A S	S W P D T Y	N E Q F F G P G T R L T V L	E D
L	BV7S8		2S5	2
	TGT GCC AGC	AGC TTT CAC AGC	CAA GAG ACC CAG TAC TTC GGG CCA GGC ACG CGG CTC CTG GTG CTC	GAG GAC
	C A S	S F H S	Q E T Q Y F G P G T R L L V L	E D
5H13	BV18S1		2S7	2
	TGT GCC AGC	TCA CCT CCT GGG TAC	TCC TAC GAG CAG TAC TTC GGG CCG GGC ACC AGG CTC ACG GTC ACA	GAG GAC
	C A S	S P P G Y	S Y E Q Y F G P G T R L T V T	E D

Clone group	V beta	N-D-N region	J beta	C beta
A	BV18S1		2S1	2
	TGT GCC AGC	TCC CGG ACT AGC GGG GCT AAC	AAT GAG CAG TTC TTC GGG CCA GGG ACA CGG CTC ACC GTG CTA	GAG GAC
	C A S	S R T S G A N	N E Q F F G P G T R L T V L	E D
B	BV7S8		2S7	2
	TGT GCC AGC	AGC GCA GTA GCG GGG ACC	TCC TAC GAG CAG TAC TTC GGG CCG GGC ACC AGG CTC ACG GTC ACA	GAG GAC
	C A S	S A V A G T	S Y E Q Y F G P G T R L T V T	E D
C	BV7S8		1S4	1
	TGT GCC AGC	AGC TTA GGC CTT TGG GGG AC GAT AAT	GAA AAA CTG TTT TTT GGC AGT GGA ACC CAG CTC TCT GTC TTG	GAG GAC
	C A S	S L G L W G T D N	E K L F F G S G T Q L S V L	E D
D	BV18S1		2S2	2
	TGT GCC AGC	TCC CCG CCC AGC GGG GGT TCG	ACC GGG GAG CTG TTT TTT GGA GAA GGC TCT AGG CTG ACC GTA CTG	GAG GAC
	C A S	S P P S G G S	T G E L F F G E G S R L T V L	E D
E	BV7S8		2S2	2
	TGT GCC AGC	AGA AAA GCG GGA CAG GAA AAC	ACC GGG GAG CTG TTT TTT GGA GAA GGC TCT AGG CTG ACC GTA CTG	GAG GAC
	C A S	R K A G Q E N	T G E L F F G E G S R L T V L	E D
F	BV7S8		2S3	2
	TGT GCC AGC	TTA TTT CCC GGT CAG GAC AGG GGA CAC GGG ACT AGC GGC AGC ACA GAT	ACG CAG TAT TTT GGC CCA GGC ACC CGG CTG ACA GTG CTC	GAG GAC
	C A S	L F P G Q D R G H G T S G S T D	T Q Y F G P G T R L T V L	E D
G	BV7S8		2S1	2
	TGT GCC AGC	AGC TTA GTA CCG	AAT GAG CAG TTC TTC GGG CCA GGG ACA CGG CTC ACC GTG CTA	GAG GAC
	C A S	S L V P	N E Q F F G P G T R L T V L	E D
H	BV18S1		2S1	2
	TGT GCC AGC	TCA CCA CTG GGC TCC TAC	AAT GAG CAG TTC TTC GGG CCA GGG ACA CGG CTC ACC GTG CTA	GAG GAC
	C A S	S P L G S Y	N E Q F F G P G T R L T V L	E D
I	BV7S8		2S3	2
	TGT GCC AGC	AGC CCC CCT AGC GGG AGG TAT	ACG CAG TAT TTT GGC CCA GGC ACC CGG CTG ACA GTG CTC	GAG GAC
	C A S	S P P S G R Y	T Q Y F G P G T R L T V L	E D
	BV10S3		1S1	1
	TGT GCC ATC	AGT GAG TCG CGA ACA GGG ATC GCT	GAA GCT TTC TTT GGA CAA GGC ACC AGA CTC ACA GTT GTA	GAG GAC
	C A I	S E S R T G I A	E A F F G Q G T R L T V V	E D
J	BV18S1		1S6	1
	TGT GCC AGC	TCA CCG ACA GGG GGG TCG CGA	TCA CCC CTC CAC TTT GGG AAC GGG ACC AGG CTC ACT GTG ACA	GAG GAC
	C A S	S P T G G S R	S P L H F G N G T R L T V T	E D
K	BV7S8		2S1	2
	TGT GCC AGC	AGC TGG CCG GAC ACC TAC	AAT GAG CAG TTC TTC GGG CCA GGG ACA CGG CTC ACC GTG CTA	GAG GAC
	C A S	S W P D T Y	N E Q F F G P G T R L T V L	E D
L	BV7S8		2S5	2
	TGT GCC AGC	AGC TTT CAC AGC	CAA GAG ACC CAG TAC TTC GGG CCA GGC ACG CGG CTC CTG GTG CTC	GAG GAC
	C A S	S F H S	Q E T Q Y F G P G T R L L V L	E D
5H13	BV18S1		2S7	2
	TGT GCC AGC	TCA CCT CCT GGG TAC	TCC TAC GAG CAG TAC TTC GGG CCG GGC ACC AGG CTC ACG GTC ACA	GAG GAC
	C A S	S P P G Y	S Y E Q Y F G P G T R L T V T	E D

Only the last 3 residues of the V region are shown, followed by the N-D-N region, the J region, and the first 2 residues of the C region. T-cell clone 5H13, also specific for the mHag HA-2, was derived from an unrelated donor.

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