Chemokine receptor expression in fresh and IPP-treated peripheral blood γδ T cells
. | Freshly isolated cells* . | . | IPP-activated cells, 36 to 50 h . | . | ||
|---|---|---|---|---|---|---|
| Chemokine receptor . | n . | % of positive cells,†mean ± SD . | n . | % of positive cells, mean ± SD . | ||
| CXCR3 | 10 | 66.5 ± 8.2 | 9 | 65.9 ± 18.9 | ||
| CXCR4 | 7 | 53.1 ± 13.8 | 6 | 57.2 ± 21.6 | ||
| CXCR5 | 7 | 2.3 ± 1.8 | 5 | 2.0 ± 2.9 | ||
| CCR1 | 5 | 20.6 ± 10.7 | 4 | 24.5 ± 16.9 | ||
| CCR2 | 9 | 23.0 ± 7.2 | 7 | 14.1 ± 5.3 | ||
| CCR4 | 12 | 13.3 ± 10.2 | 12 | 29.9 ± 10.8 | ||
| CCR5 | 12 | 61.2 ± 10.4 | 9 | 2.5 ± 5.4 | ||
| CCR6 | 6 | 20.1 ± 11.1 | 4 | 15.2 ± 14.7 | ||
| CCR7 | 23 | 18.9 ± 9.7 | 14 | 77.6 ± 14.7 | ||
. | Freshly isolated cells* . | . | IPP-activated cells, 36 to 50 h . | . | ||
|---|---|---|---|---|---|---|
| Chemokine receptor . | n . | % of positive cells,†mean ± SD . | n . | % of positive cells, mean ± SD . | ||
| CXCR3 | 10 | 66.5 ± 8.2 | 9 | 65.9 ± 18.9 | ||
| CXCR4 | 7 | 53.1 ± 13.8 | 6 | 57.2 ± 21.6 | ||
| CXCR5 | 7 | 2.3 ± 1.8 | 5 | 2.0 ± 2.9 | ||
| CCR1 | 5 | 20.6 ± 10.7 | 4 | 24.5 ± 16.9 | ||
| CCR2 | 9 | 23.0 ± 7.2 | 7 | 14.1 ± 5.3 | ||
| CCR4 | 12 | 13.3 ± 10.2 | 12 | 29.9 ± 10.8 | ||
| CCR5 | 12 | 61.2 ± 10.4 | 9 | 2.5 ± 5.4 | ||
| CCR6 | 6 | 20.1 ± 11.1 | 4 | 15.2 ± 14.7 | ||
| CCR7 | 23 | 18.9 ± 9.7 | 14 | 77.6 ± 14.7 | ||
Chemokine receptor expression was analyzed in freshly isolated as well as in cultured γδ T cells that were stimulated with IPP for 36 hours to 50 hours.
Chemokine receptor positivity by flow cytometric analysis was expressed as mean plus SD of the fraction of positive cells in percent, and n refers to the number of donors analyzed. Percent positivity equals the fraction of antibody-stained cells after substraction of the isotype-matched control stainings.