LCLs express higher levels of immunostimulatory molecules than PBMCs
Marker . | PBMCs, % positive . | PBMCs, MFI . | LCLs, % positive . | LCLs, MFI . |
---|---|---|---|---|
HLA class 1 | 96.6 ± 4.2 | 185.5 ± 159.5 | 97.4 ± 2.8 | 334.4 ± 146.3 |
HLA-DR | 31.5 ± 4.0 | 181.4 ± 127.4 | 99.7 ± 0.2 | 1904.8 ± 545 |
CD40 | 3.9 ± 5.0 | 3.3 ± 1.4 | 42.4 ± 8.0 | 36 ± 18.6 |
CD54 | 25.4 ± 12.3 | 28.8 ± 28.5 | 95.4 ± 2.3 | 393.6 ± 203.7 |
CD80 | 1.0 ± 0.8 | 2.2 ± 0.5 | 95.1 ± 3.6 | 206.8 ± 135 |
CD86 | 10.4 ± 4.0 | 8.3 ± 2.2 | 81.3 ± 5.5 | 184.1 ± 132 |
Marker . | PBMCs, % positive . | PBMCs, MFI . | LCLs, % positive . | LCLs, MFI . |
---|---|---|---|---|
HLA class 1 | 96.6 ± 4.2 | 185.5 ± 159.5 | 97.4 ± 2.8 | 334.4 ± 146.3 |
HLA-DR | 31.5 ± 4.0 | 181.4 ± 127.4 | 99.7 ± 0.2 | 1904.8 ± 545 |
CD40 | 3.9 ± 5.0 | 3.3 ± 1.4 | 42.4 ± 8.0 | 36 ± 18.6 |
CD54 | 25.4 ± 12.3 | 28.8 ± 28.5 | 95.4 ± 2.3 | 393.6 ± 203.7 |
CD80 | 1.0 ± 0.8 | 2.2 ± 0.5 | 95.1 ± 3.6 | 206.8 ± 135 |
CD86 | 10.4 ± 4.0 | 8.3 ± 2.2 | 81.3 ± 5.5 | 184.1 ± 132 |
Data are the results of FACS analysis of PBMCs or LCLs from the same individuals. The results are the means ± SDs of 5 healthy donors.
MFI indicates mean fluorescence intensity.