Table 1.

LCLs express higher levels of immunostimulatory molecules than PBMCs


Marker

PBMCs, % positive

PBMCs, MFI

LCLs, % positive

LCLs, MFI
HLA class 1   96.6 ± 4.2   185.5 ± 159.5   97.4 ± 2.8   334.4 ± 146.3  
HLA-DR   31.5 ± 4.0   181.4 ± 127.4   99.7 ± 0.2   1904.8 ± 545  
CD40   3.9 ± 5.0   3.3 ± 1.4   42.4 ± 8.0   36 ± 18.6  
CD54   25.4 ± 12.3   28.8 ± 28.5   95.4 ± 2.3   393.6 ± 203.7  
CD80   1.0 ± 0.8   2.2 ± 0.5   95.1 ± 3.6   206.8 ± 135  
CD86
 
10.4 ± 4.0
 
8.3 ± 2.2
 
81.3 ± 5.5
 
184.1 ± 132
 

Marker

PBMCs, % positive

PBMCs, MFI

LCLs, % positive

LCLs, MFI
HLA class 1   96.6 ± 4.2   185.5 ± 159.5   97.4 ± 2.8   334.4 ± 146.3  
HLA-DR   31.5 ± 4.0   181.4 ± 127.4   99.7 ± 0.2   1904.8 ± 545  
CD40   3.9 ± 5.0   3.3 ± 1.4   42.4 ± 8.0   36 ± 18.6  
CD54   25.4 ± 12.3   28.8 ± 28.5   95.4 ± 2.3   393.6 ± 203.7  
CD80   1.0 ± 0.8   2.2 ± 0.5   95.1 ± 3.6   206.8 ± 135  
CD86
 
10.4 ± 4.0
 
8.3 ± 2.2
 
81.3 ± 5.5
 
184.1 ± 132
 

Data are the results of FACS analysis of PBMCs or LCLs from the same individuals. The results are the means ± SDs of 5 healthy donors.

MFI indicates mean fluorescence intensity.

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