Selection of clones by differential screening
. | No. of clones . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|
| . | . | Screening specificity . | . | . | . | ||||
| Library origin of clones . | Total . | Nil . | Nonspecific . | Partial specificity . | Exclusive specificity . | ||||
| Small subtracted | 465 | 206 | 188 | 7 | 64 | ||||
| Large subtracted | 454 | 352 | 41 | 8 | 53 | ||||
. | No. of clones . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|
| . | . | Screening specificity . | . | . | . | ||||
| Library origin of clones . | Total . | Nil . | Nonspecific . | Partial specificity . | Exclusive specificity . | ||||
| Small subtracted | 465 | 206 | 188 | 7 | 64 | ||||
| Large subtracted | 454 | 352 | 41 | 8 | 53 | ||||
Total represents the number of clones picked for differential screening. Screening specificity indicates that plated clones were screened with each of 4 different 32P-labeled probes made from unsubtracted small- and large-cell cDNA, subtracted small minus large cDNA, and subtracted large minus small cDNA. Hybridization was measured by densitometry. Nil indicates not selected because of insufficient binding of subtracted and unsubtracted probes. Nonspecific indicates not selected because of nonspecific binding of probes derived from tester and driver cDNA. Partial specificity indicates selected for sequencing because hybridization with forward (tester) probes was at least 10-fold greater than reverse of subtracted probe and 5-fold for unsubtracted probes using densitometry. Exclusive specificity indicates selected for sequencing because hybridization occurred only with forward (tester) subtracted and unsubtracted probes.