Turnover kinetics of erythroid and megakaryocyte lineage cells
Cell type* . | n† . | % new cells, “f” × 100‡ . | Fractional replacement rates, k/d‡ . | Replacement t1/2labeling, d‡ . | Replacement t1/2delabeling, d§ . |
|---|---|---|---|---|---|
| Glycophorin A+ cells | 4 | 35.4 ± 5.31 | 0.23 ± 0.042 | 3.5 ± 0.91 | 3.3 ± 0.48 |
| CD41a+ cells | 5 | 15.2 ± 2.98 | 0.08 ± 0.014 | 10.2 ± 2.88 | 5.4 ± 2.9∥ |
Cell type* . | n† . | % new cells, “f” × 100‡ . | Fractional replacement rates, k/d‡ . | Replacement t1/2labeling, d‡ . | Replacement t1/2delabeling, d§ . |
|---|---|---|---|---|---|
| Glycophorin A+ cells | 4 | 35.4 ± 5.31 | 0.23 ± 0.042 | 3.5 ± 0.91 | 3.3 ± 0.48 |
| CD41a+ cells | 5 | 15.2 ± 2.98 | 0.08 ± 0.014 | 10.2 ± 2.88 | 5.4 ± 2.9∥ |
CD41a+/glycophorin A- and CD41a-/glycophorin A+ cells were sorted by FACS from CD34- cell fractions from marrow aspirates obtained at day 0 following 45 to 49 hours of continuous [2H2]-glucose infusion.
Number of different donors.
Values are the mean ± SEM determined calculated from measurements of %[2H2] dA enrichment as described in “Patients, materials, and methods”. The percent new cells takes into consideration the mean plasma [2H2]-glucose concentration, and calculations for fractional replacement rates (k/day) and replacement rate t1/2 also take into account the number of hours of continuous [2H2]-glucose infusion.
Calculated from the decrease in %[2H2] dA enrichment observed at week 2.
n = 3.