Comparison of in vivo labeling and delabeling kinetics of CD34+ cells, CD133+, and CD133–CD34+ cells
Donor* and selected cells† . | % Newly produced cells, “f” × 100‡ . | Fractional replacement rates, klabeling/d‡ . | Replacement t1/2labeling, d‡ . | Replacement t1/2delabeling, d§ . |
|---|---|---|---|---|
| Donor 15 | ||||
| CD133+ cells | 32.5 | 0.197 | 3.5 | 4.1 |
| CD133-CD34+ cells | 39.8 | 0.254 | 2.7 | 3.0 |
| Donor 18 | ||||
| CD133+ cells | 11.6 | 0.061 | 11.3 | 10.3 |
| CD133-CD34+ cells | 43.6 | 0.286 | 2.4 | 3.9 |
| Donor 22 | ||||
| CD133+ cells | 29.6 | 0.186 | 3.8 | ND |
| CD133-CD34+ cells | 40.4 | 0.274 | 2.5 | ND |
| Donor 14 (day 1)∥ | ||||
| CD133+ cells | 21.5 | 0.117 | 5.9 | 6.1 |
| CD133-CD34+ cells | 37.4 | 0.227 | 3.0 | 3.5 |
Donor* and selected cells† . | % Newly produced cells, “f” × 100‡ . | Fractional replacement rates, klabeling/d‡ . | Replacement t1/2labeling, d‡ . | Replacement t1/2delabeling, d§ . |
|---|---|---|---|---|
| Donor 15 | ||||
| CD133+ cells | 32.5 | 0.197 | 3.5 | 4.1 |
| CD133-CD34+ cells | 39.8 | 0.254 | 2.7 | 3.0 |
| Donor 18 | ||||
| CD133+ cells | 11.6 | 0.061 | 11.3 | 10.3 |
| CD133-CD34+ cells | 43.6 | 0.286 | 2.4 | 3.9 |
| Donor 22 | ||||
| CD133+ cells | 29.6 | 0.186 | 3.8 | ND |
| CD133-CD34+ cells | 40.4 | 0.274 | 2.5 | ND |
| Donor 14 (day 1)∥ | ||||
| CD133+ cells | 21.5 | 0.117 | 5.9 | 6.1 |
| CD133-CD34+ cells | 37.4 | 0.227 | 3.0 | 3.5 |
Marrow aspirates were obtained within 2 hours of discontinuing a 45-49 hour continuous [2H2]-glucose.
CD133+ cells were selected from low-density marrow cells, and then CD34+ cells were selected from the CD133- cell fraction (CD133-CD34+ cells).
Values are the means determined from 3 measurements for %[2H2] dA enrichment from the same sample of processed cells. The fraction of new cells (F) and kinetic parameters were calculated as described in “Patients, materials, and methods”.19,21
Calculated from the decrease in %[2H2] dA enrichment observed at week 2.
Estimated from marrow samples obtained 24 hours after the end of the [2H2]-glucose infusion period. The %[2H2] dA enrichment was 3.1 ± 0.04 for CD133+ cells, 5.4 ± 0.06 for CD133-CD34+ cells, and 5.1 ± 0.09 for CD34+ cells from the same marrow sample.