Enrichment of normal donor marrow cells for CD34+ and CD133+ cells
Source of marrow cells* . | % CD34+ cells (n)† . | % CD34+ cells coexpressing CD133 (n)† . |
|---|---|---|
| Unseparated cells | 1.8 ± 0.16 (27)‡ | 33.7 ± 1.39 (24)§ |
| (A) CD34+ selected cells | 96.6 ± 0.49 (27)‡ | 38.6 ± 2.10 (24)§ |
| (B) CD133+ selected cells | 96.8 ± 0.23 (8) | 80.8 ± 1.51 (8) |
| (C) CD133-/dimCD34+ cells | 98.1 ± 0.31 (6) | 12.1 ± 1.10 (6) |
Source of marrow cells* . | % CD34+ cells (n)† . | % CD34+ cells coexpressing CD133 (n)† . |
|---|---|---|
| Unseparated cells | 1.8 ± 0.16 (27)‡ | 33.7 ± 1.39 (24)§ |
| (A) CD34+ selected cells | 96.6 ± 0.49 (27)‡ | 38.6 ± 2.10 (24)§ |
| (B) CD133+ selected cells | 96.8 ± 0.23 (8) | 80.8 ± 1.51 (8) |
| (C) CD133-/dimCD34+ cells | 98.1 ± 0.31 (6) | 12.1 ± 1.10 (6) |
Measurements from unseparated cells are from marrow aspirates before any manipulation. Marrows were separated over a Ficoll gradient, and the low-density cells were enriched for either CD34+ cells (A) or CD133+ cells (B) by positive immunomagnetic selection, and (C) after selection of CD133+ cells, the negative fraction was enriched for CD34+ cells (CD133-/dimCD34+ cells).
Number of marrow samples. Values are mean ± SEM percentages determined from flow cytometry analyses of cells obtained from donors 1 to 3 different times after completion of [2H2]-glucose infusion.
Paired for the same donors.
Paired for the same donors, by paired t test, P = .96.