Effects of antibodies on colony formation in methylcellulose cultures
Antibody . | Number of CFU-Cs per 105 BM nucleated cells . |
|---|---|
| Rat IgG | 258 ± 19 |
| Anti-α4 integrin (PS/2) | 253 ± 20 |
| Anti-PSGL-1 (4RA10) | 257 ± 18 |
| Anti-α4 integrin + anti-PSGL-1 | 243 ± 18 |
Antibody . | Number of CFU-Cs per 105 BM nucleated cells . |
|---|---|
| Rat IgG | 258 ± 19 |
| Anti-α4 integrin (PS/2) | 253 ± 20 |
| Anti-PSGL-1 (4RA10) | 257 ± 18 |
| Anti-α4 integrin + anti-PSGL-1 | 243 ± 18 |
Wild-type donor bone marrow cells were incubated with indicated antibodies (2 μg/106 nucleated cells) for 3 hours and plated for CFU-C assays. Antibody treatment did not alter colony formation. Data are shown as means ± SEMs; n = 7.