TNF-α and IFN-γ intracytoplasmic expression in marrow MNCs in 7 FA patients and 8 controls
. | FA patients . | . | . | Controls . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
. | Mean (SD) . | Median . | Min-Max . | Mean (SD) . | Median . | Min-Max . | P* . | ||||
| TNF-α | |||||||||||
| TNF-α+/CD3+ cells, %† | 9.57 (8.68) | 7 | 0-21 | 0.00 (0.00) | 0 | 0-0 | .006 | ||||
| TNF-α+/CD3+ cells, MRFI | 3.71 (3.09) | 2 | 1-8 | 1.00 (0.00) | 1 | 1-1 | .006 | ||||
| TNF-α+/CD3-, % | 6.29 (6.57) | 4 | 0-19 | 0.50 (0.53) | 0.5 | 0-1 | .016 | ||||
| TNF-α+/CD3-, MRFI | 1.86 (0.90) | 2 | 1-3 | 1.06 (0.18) | 1 | 1-1.5 | .045 | ||||
| IFN-γ | |||||||||||
| IFN-γ+/CD3+ cells, % | 8.57 (7.91) | 9 | 0-20 | 0.25 (0.71) | 0 | 0-2 | .013 | ||||
| IFN-γ+/CD3+ cells, MRFI | 2.29 (1.25) | 2 | 1-4 | 1.12 (0.35) | 1 | 1-2 | .021 | ||||
| IFN-γ+/CD3-, % | 5.57 (7.07) | 3 | 0-20 | 0.37 (0.74) | 0 | 0-2 | .014 | ||||
| IFN-γ+/CD3-, MRFI | 2.00 (2.65) | 1 | 1-8 | 1.00 (0.00) | 1 | 1-1 | |||||
. | FA patients . | . | . | Controls . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
. | Mean (SD) . | Median . | Min-Max . | Mean (SD) . | Median . | Min-Max . | P* . | ||||
| TNF-α | |||||||||||
| TNF-α+/CD3+ cells, %† | 9.57 (8.68) | 7 | 0-21 | 0.00 (0.00) | 0 | 0-0 | .006 | ||||
| TNF-α+/CD3+ cells, MRFI | 3.71 (3.09) | 2 | 1-8 | 1.00 (0.00) | 1 | 1-1 | .006 | ||||
| TNF-α+/CD3-, % | 6.29 (6.57) | 4 | 0-19 | 0.50 (0.53) | 0.5 | 0-1 | .016 | ||||
| TNF-α+/CD3-, MRFI | 1.86 (0.90) | 2 | 1-3 | 1.06 (0.18) | 1 | 1-1.5 | .045 | ||||
| IFN-γ | |||||||||||
| IFN-γ+/CD3+ cells, % | 8.57 (7.91) | 9 | 0-20 | 0.25 (0.71) | 0 | 0-2 | .013 | ||||
| IFN-γ+/CD3+ cells, MRFI | 2.29 (1.25) | 2 | 1-4 | 1.12 (0.35) | 1 | 1-2 | .021 | ||||
| IFN-γ+/CD3-, % | 5.57 (7.07) | 3 | 0-20 | 0.37 (0.74) | 0 | 0-2 | .014 | ||||
| IFN-γ+/CD3-, MRFI | 2.00 (2.65) | 1 | 1-8 | 1.00 (0.00) | 1 | 1-1 | |||||
Min indicates minimum; max, maximum.
Mann-Whitney U test; only statistically significant P values are shown.
CD3 phenotypically defined cells were chosen because they contain the cell fraction that is known to produce these cytokines in healthy subjects and in marrow failure disease such as AA.10-12