Primers used for genomic amplification of P5N-I gene exons
Exon . | Primer . | PCR product, bp . |
|---|---|---|
| 3 | F- 5′ AACTAATAATTAATTTTCTTTTAC 3′ | 183 |
| R- 5′ GAATTACAGGCATGAGCCAA 3′ | ||
| 4 | F- 5′ GAGAAATTCTGGCCATGTGG 3′ | 204 |
| R- 5′ CACAGGTAATATAAAGAGGATC 3′ | ||
| 5 | F- 5′ GCAAATTATTCATAGTAGTGG 3′ | 204 |
| R- 5′ CTTCTGTGAATGCACCTGAAG 3′ | ||
| 6 | F- 5′ GCTTTCTATAAAACCAAGCAG 3′ | 226 |
| R- 5′ GCCATCAGTTGTAACTGCAG 3′ | ||
| 7 | F- 5′ CTCACATGGAAGAATGTTTTG 3′ | 230 |
| R- 5′ GCAATAATTCAAGTCTGTGAAC 3′ | ||
| 8 | F- 5′ GCTTCTAAGTAAAAAGGAAC 3′ | 325 |
| R- 5′ CTGGATATAGGATATATATTAAG 3′ | ||
| 9 | F- 5′ ATAGAATTTTTAAAGCCT GTT C 3′ | 306 |
| R- 5′ TTGCCTATCAAGTTTCATCC 3′ | ||
| 10 | F- 5′ TTGTTTCTTTGCCCTGCATG 3′ | 305 |
| R-5′ GTCAATGCATTCACCATATC 3′ |
Exon . | Primer . | PCR product, bp . |
|---|---|---|
| 3 | F- 5′ AACTAATAATTAATTTTCTTTTAC 3′ | 183 |
| R- 5′ GAATTACAGGCATGAGCCAA 3′ | ||
| 4 | F- 5′ GAGAAATTCTGGCCATGTGG 3′ | 204 |
| R- 5′ CACAGGTAATATAAAGAGGATC 3′ | ||
| 5 | F- 5′ GCAAATTATTCATAGTAGTGG 3′ | 204 |
| R- 5′ CTTCTGTGAATGCACCTGAAG 3′ | ||
| 6 | F- 5′ GCTTTCTATAAAACCAAGCAG 3′ | 226 |
| R- 5′ GCCATCAGTTGTAACTGCAG 3′ | ||
| 7 | F- 5′ CTCACATGGAAGAATGTTTTG 3′ | 230 |
| R- 5′ GCAATAATTCAAGTCTGTGAAC 3′ | ||
| 8 | F- 5′ GCTTCTAAGTAAAAAGGAAC 3′ | 325 |
| R- 5′ CTGGATATAGGATATATATTAAG 3′ | ||
| 9 | F- 5′ ATAGAATTTTTAAAGCCT GTT C 3′ | 306 |
| R- 5′ TTGCCTATCAAGTTTCATCC 3′ | ||
| 10 | F- 5′ TTGTTTCTTTGCCCTGCATG 3′ | 305 |
| R-5′ GTCAATGCATTCACCATATC 3′ |
F indicates forward; R, reverse.