Immunophenotype of culture-expanded second-trimester MSCs: expression of antigens on cultured MSC-derived from amniotic fluid
Antigen . | CD no. . | Expression . |
|---|---|---|
| Mesenchymal | ||
| Thy-1 | CD90d | + |
| Endoglin, SH2 | CD105e | + |
| SH3, SH4a | CD73 | + |
| SB10/ALCAM | CD166f | + |
| HLA | ||
| HLA ABCb | None | + |
| HLA DRc | None | - |
| Integrins | ||
| VLA-4 | CD49dg | - |
| VLA-5 | CD49eg | + |
| LFA-1 | CD11ac | - |
| Selectins | ||
| E-selectins | CD62Eh | - |
| L-selectins | CD62Lh | - |
| P-selectins | CD62Ph | - |
| Hematopoietic | ||
| LCA | CD45c | - |
| gp 105-120 | CD34c | - |
| LPS-R | CD14c | - |
| Ig superfamily | ||
| PECAM | CD31i | - |
| LFA-3 | CD58j | ± |
| ICAM-1 | CD54f | + |
| ICAM-3 | CD50d | - |
| VCAM-1 | CD106k | - |
| B7.1 | CD80g | - |
| B7.2 | CD86d | - |
| Cytokine receptors | ||
| IL-2 receptor | CD25c | - |
| IL-3 receptor | CD123d | ± |
| IL-7 receptor | CD127g | - |
| TNF-α1 receptor | CD120ak | - |
| TNF-α2 receptor | CD120bk | - |
| Other antigens | ||
| Transferrin receptor | CD71f | ± |
| HCAM-1 | CD44d | + |
| Bp50 | CD40l | - |
Antigen . | CD no. . | Expression . |
|---|---|---|
| Mesenchymal | ||
| Thy-1 | CD90d | + |
| Endoglin, SH2 | CD105e | + |
| SH3, SH4a | CD73 | + |
| SB10/ALCAM | CD166f | + |
| HLA | ||
| HLA ABCb | None | + |
| HLA DRc | None | - |
| Integrins | ||
| VLA-4 | CD49dg | - |
| VLA-5 | CD49eg | + |
| LFA-1 | CD11ac | - |
| Selectins | ||
| E-selectins | CD62Eh | - |
| L-selectins | CD62Lh | - |
| P-selectins | CD62Ph | - |
| Hematopoietic | ||
| LCA | CD45c | - |
| gp 105-120 | CD34c | - |
| LPS-R | CD14c | - |
| Ig superfamily | ||
| PECAM | CD31i | - |
| LFA-3 | CD58j | ± |
| ICAM-1 | CD54f | + |
| ICAM-3 | CD50d | - |
| VCAM-1 | CD106k | - |
| B7.1 | CD80g | - |
| B7.2 | CD86d | - |
| Cytokine receptors | ||
| IL-2 receptor | CD25c | - |
| IL-3 receptor | CD123d | ± |
| IL-7 receptor | CD127g | - |
| TNF-α1 receptor | CD120ak | - |
| TNF-α2 receptor | CD120bk | - |
| Other antigens | ||
| Transferrin receptor | CD71f | ± |
| HCAM-1 | CD44d | + |
| Bp50 | CD40l | - |
+ indicates positive; -, negative; VLA, very late antigen; LFA, leukocyte function antigen; LCA, leukocyte common antigen; LPS-R, lipopolysacharid-receptor; PECAM, platelet-endothelial cell adhesion molecule; ±, weakly positive; VCAM-1, vascular cell endothelial molecule; and HCAM-1, homing cellular adhesion molecule.
A gift of Dr. A. Moseley, Osiris Therapies, Baltimore, MD.
Instruchemie, Hilversum, the Netherlands.
Beckton Dickinson, San Jose, CA.
PharMingen, San Diego, CA.
Ancell, Bayport, MN.
CLB, Amsterdam, the Netherlands.
Immunotech Coulter, Marseille, France.
Bio-Whittaker, Verviers, Belgium.
DAKO, Glostrup, Denmark.
Southern Biotechnology Associates, Birmingham, AL.
R&D Systems, Abingdon, United Kingdom.
Serotec, Oxford, United Kingdom.