Reduced SDF-1—induced chemotaxis of WASP-deficient bone marrow cells
Bone marrow cells . | Without SDF-1, % . | With SDF-1, % . |
|---|---|---|
| Wild-type mice | 3.3 ± 1.3 | 28.2 ± 4.4 |
| WASP-deficient mice | 1.1 ± 0.4 | 13.7 ± 3.3 |
| 5-FU wild-type mice | 5.4 | 36.0 |
| 5-FU WASP-deficient mice | 1.5 | 16.1 |
Bone marrow cells . | Without SDF-1, % . | With SDF-1, % . |
|---|---|---|
| Wild-type mice | 3.3 ± 1.3 | 28.2 ± 4.4 |
| WASP-deficient mice | 1.1 ± 0.4 | 13.7 ± 3.3 |
| 5-FU wild-type mice | 5.4 | 36.0 |
| 5-FU WASP-deficient mice | 1.5 | 16.1 |
SDF-1—induced chemotaxis of total bone marrow cells or of bone marrow cells from 5-FU—treated mice was assayed in transwell chemotaxis chambers. First, 2.5 × 105 cells suspended in culture medium were placed in the upper chamber and allowed to migrate for 1 hour at 37°C. Cells migrating to the lower chamber containing SDF-1 were counted and expressed as the ratio of the number of migrating cells to the number of cells placed in the upper chamber. Data are means ± SD; n = 4 for the total bone marrow cell experiments and n = 2 for experiments with bone marrow from 5-FU—treated mice.