NKG2DL expression and release by patient leukemia cells
UPN . | MICA, pg/mL . | MICB, pg/mL . | ULBP1, SFI . | ULBP2, SFI . | ULBP3, SFI . | sMICA, pg/mL . | nMICA, pg/mL . | sMICB, SFI . | nMICB, SFI . |
|---|---|---|---|---|---|---|---|---|---|
| 1 | 5.0 | 1.8 | 1.0 | 8.5 | 8.2 | 924 | 9.2 | 288 | 2.9 |
| 2 | 3.1 | 3.2 | 2.3 | 1.1 | 1.0 | 885 | 7.5 | 36 | 0.3 |
| 3 | 1.5 | 1.0 | 1.0 | 1.1 | 1.0 | 267 | 6.9 | 336 | 8.6 |
| 4 | 7.3 | 1.2 | 1.0 | 1.0 | 1.0 | 10 296 | 591.7 | 27 | 1.6 |
| 5 | 3.2 | 1.2 | 1.0 | 1.1 | 1.1 | 978 | 11.2 | 183 | 2.1 |
| 6 | 1.4 | 1.5 | 1.3 | 1.7 | 1.0 | 54 | 0.8 | 90 | 1.3 |
| 7 | 1.1 | 1.0 | 1.0 | 1.1 | 1.1 | 75 | 8.3 | 72 | 8.0 |
| 8 | 1.1 | 1.3 | 1.0 | 1.0 | 1.0 | 99 | 110.0 | 126 | 140.0 |
| 9 | 1.2 | 1.6 | 1.7 | 1.7 | 1.0 | 318 | 454.3 | 150 | 214.3 |
| 10 | 1.1 | 10.0 | 1.3 | 1.0 | 1.1 | ND | ND | ND | ND |
| 11 | 2.7 | 1.7 | 1.0 | 3.3 | 1.6 | ND | ND | ND | ND |
| 12 | 2.8 | 1.0 | 1.0 | 1.0 | 2.4 | 642 | 401.3 | 132 | 82.5 |
| 13 | 1.1 | 1.0 | 1.0 | 1.1 | 1.0 | 216 | 6.4 | 180 | 5.3 |
| 14 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 141 | 10.8 | 57 | 4.4 |
| 15 | 1.5 | 1.2 | 1.1 | 1.1 | 1.0 | 114 | 1.8 | 69 | 1.1 |
| 16 | 2.2 | 2.1 | 1.1 | 1.0 | 1.1 | 174 | 13.5 | 69 | 5.3 |
| 17 | 1.4 | 1.2 | 6.4 | 1.9 | 1.0 | 159 | 1.3 | 117 | 0.9 |
| 18 | 4.7 | 4.8 | 3.9 | 1.0 | 6.7 | 321 | 267.5 | 303 | 252.5 |
| 19 | 12.1 | 3.6 | 1.1 | 1.0 | 1.8 | 549 | 51.0 | 66 | 6.2 |
| 20 | 5.6 | 3.1 | 1.9 | 1.8 | 1.0 | 1 173 | 24.4 | 69 | 1.4 |
| 21 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 96 | 0.5 | 153 | 0.8 |
| 22 | 1.7 | 1.3 | 1.8 | 1.0 | 1.3 | 150 | 1.2 | 318 | 2.5 |
| 23 | 1.0 | 1.0 | 1.0 | 1.0 | 2.7 | 216 | 19.6 | 288 | 26.2 |
| 24 | 6.9 | 5.2 | 8.1 | 8.1 | 2.3 | ND | ND | ND | ND |
| 25 | 1.2 | 1.0 | 1.1 | 1.0 | 1.3 | ND | ND | ND | ND |
UPN . | MICA, pg/mL . | MICB, pg/mL . | ULBP1, SFI . | ULBP2, SFI . | ULBP3, SFI . | sMICA, pg/mL . | nMICA, pg/mL . | sMICB, SFI . | nMICB, SFI . |
|---|---|---|---|---|---|---|---|---|---|
| 1 | 5.0 | 1.8 | 1.0 | 8.5 | 8.2 | 924 | 9.2 | 288 | 2.9 |
| 2 | 3.1 | 3.2 | 2.3 | 1.1 | 1.0 | 885 | 7.5 | 36 | 0.3 |
| 3 | 1.5 | 1.0 | 1.0 | 1.1 | 1.0 | 267 | 6.9 | 336 | 8.6 |
| 4 | 7.3 | 1.2 | 1.0 | 1.0 | 1.0 | 10 296 | 591.7 | 27 | 1.6 |
| 5 | 3.2 | 1.2 | 1.0 | 1.1 | 1.1 | 978 | 11.2 | 183 | 2.1 |
| 6 | 1.4 | 1.5 | 1.3 | 1.7 | 1.0 | 54 | 0.8 | 90 | 1.3 |
| 7 | 1.1 | 1.0 | 1.0 | 1.1 | 1.1 | 75 | 8.3 | 72 | 8.0 |
| 8 | 1.1 | 1.3 | 1.0 | 1.0 | 1.0 | 99 | 110.0 | 126 | 140.0 |
| 9 | 1.2 | 1.6 | 1.7 | 1.7 | 1.0 | 318 | 454.3 | 150 | 214.3 |
| 10 | 1.1 | 10.0 | 1.3 | 1.0 | 1.1 | ND | ND | ND | ND |
| 11 | 2.7 | 1.7 | 1.0 | 3.3 | 1.6 | ND | ND | ND | ND |
| 12 | 2.8 | 1.0 | 1.0 | 1.0 | 2.4 | 642 | 401.3 | 132 | 82.5 |
| 13 | 1.1 | 1.0 | 1.0 | 1.1 | 1.0 | 216 | 6.4 | 180 | 5.3 |
| 14 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 141 | 10.8 | 57 | 4.4 |
| 15 | 1.5 | 1.2 | 1.1 | 1.1 | 1.0 | 114 | 1.8 | 69 | 1.1 |
| 16 | 2.2 | 2.1 | 1.1 | 1.0 | 1.1 | 174 | 13.5 | 69 | 5.3 |
| 17 | 1.4 | 1.2 | 6.4 | 1.9 | 1.0 | 159 | 1.3 | 117 | 0.9 |
| 18 | 4.7 | 4.8 | 3.9 | 1.0 | 6.7 | 321 | 267.5 | 303 | 252.5 |
| 19 | 12.1 | 3.6 | 1.1 | 1.0 | 1.8 | 549 | 51.0 | 66 | 6.2 |
| 20 | 5.6 | 3.1 | 1.9 | 1.8 | 1.0 | 1 173 | 24.4 | 69 | 1.4 |
| 21 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 96 | 0.5 | 153 | 0.8 |
| 22 | 1.7 | 1.3 | 1.8 | 1.0 | 1.3 | 150 | 1.2 | 318 | 2.5 |
| 23 | 1.0 | 1.0 | 1.0 | 1.0 | 2.7 | 216 | 19.6 | 288 | 26.2 |
| 24 | 6.9 | 5.2 | 8.1 | 8.1 | 2.3 | ND | ND | ND | ND |
| 25 | 1.2 | 1.0 | 1.1 | 1.0 | 1.3 | ND | ND | ND | ND |
After isolation, malignant cells were selected by staining with anti-CD33, CD34, or CD19 and stained for the indicated NKG2DL. Expression was quantified as specific fluorescence index (SFI) values as described in “Patients, materials, and methods.” Levels of sMICA and sMICB molecules were determined as described in “Patients, materials, and methods.” Serum levels were then divided by the respective white blood count of each patient and are shown as normalized MICA and MICB (nMICA, nMICB). ND indicates not done.