Kinetics of interaction between immobilized p97 and mAbs
Antibody . | ΔRU . | Ka, M-1 s-1 . | Kd, s-1 . | KA = Ka/Kd, M-1 . | KD = Kd/Ka, M . |
|---|---|---|---|---|---|
| L235 | 1055 ± 82 | 4.4 × 104 | 5.3 × 10-5 | 0.9 × 109 | 0.1 × 10-10 |
| HybC | 1509 ± 184 | 7.2 × 104 | 4.5 × 10-5 | 1.6 × 109 | 6.4 × 10-10 |
| HybE | 232 ± 52 | 0.9 × 104 | 9.8 × 10-5 | 0.08 × 109 | 0.01 × 10-10 |
| HybF | 2199 ± 150 | 8.0 × 104 | 3.0 × 10-5 | 2.7 × 109 | 3.8 × 10-10 |
| 9B6 | 2440 ± 112 | 1.2 × 104 | 9.1 × 10-5 | 1.3 × 109 | 7.9 × 10-10 |
| 2C7 | 2290 ± 87 | 5.9 × 104 | 3.8 × 10-5 | 1.6 × 109 | 6.5 × 10-10 |
Antibody . | ΔRU . | Ka, M-1 s-1 . | Kd, s-1 . | KA = Ka/Kd, M-1 . | KD = Kd/Ka, M . |
|---|---|---|---|---|---|
| L235 | 1055 ± 82 | 4.4 × 104 | 5.3 × 10-5 | 0.9 × 109 | 0.1 × 10-10 |
| HybC | 1509 ± 184 | 7.2 × 104 | 4.5 × 10-5 | 1.6 × 109 | 6.4 × 10-10 |
| HybE | 232 ± 52 | 0.9 × 104 | 9.8 × 10-5 | 0.08 × 109 | 0.01 × 10-10 |
| HybF | 2199 ± 150 | 8.0 × 104 | 3.0 × 10-5 | 2.7 × 109 | 3.8 × 10-10 |
| 9B6 | 2440 ± 112 | 1.2 × 104 | 9.1 × 10-5 | 1.3 × 109 | 7.9 × 10-10 |
| 2C7 | 2290 ± 87 | 5.9 × 104 | 3.8 × 10-5 | 1.6 × 109 | 6.5 × 10-10 |
The difference between the relative units measured after and before injection of mAbs directed against p97 are presented (ΔRU) as well as the apparent association (Ka) and dissociation (Kd) constants. The affinity (KA) and dissociation (KD) constants were calculated from the Ka and Kd.