Table 4.

Reactivity of patient sera with CHO cells expressing GPIbαβ and wild-type or chimeric GPIX proteins

% cells positive in the presence of quinine
CHOαβ (+)
Patient 1
Patient 2
Patient 3
Patient 4
Patient 5
Patient 6
WT GPIX   97.32   78.55   58.31   69.90   81.34   45.76  
Chimera 1   81.27   61.03   54.30   42.68   85.68   36.07  
Chimera 2   91.45   62.81   50.62   53.38   92.94   24.90  
Chimera 3   3.30   4.47   11.66   9.88   5.24   0.20  
Chimera 4
 		 62.19
 		 88.33
 		 65.02
 		 75.76
 		 89.86
 		 48.98
 
% cells positive in the presence of quinine
CHOαβ (+)
Patient 1
Patient 2
Patient 3
Patient 4
Patient 5
Patient 6
WT GPIX   97.32   78.55   58.31   69.90   81.34   45.76  
Chimera 1   81.27   61.03   54.30   42.68   85.68   36.07  
Chimera 2   91.45   62.81   50.62   53.38   92.94   24.90  
Chimera 3   3.30   4.47   11.66   9.88   5.24   0.20  
Chimera 4
 		 62.19
 		 88.33
 		 65.02
 		 75.76
 		 89.86
 		 48.98
 

Flow cytometric analysis was performed by incubating patient sera and CHO cells expressing GPIbαβ and wild-type or chimeric GPIX glycoproteins in the presence or absence of quinine as described in “Patients, materials, and methods.” WT indicates wild-type; GPIbαβ, CHO cells expressing GPIbα and GPIbβ. In the absence of quinine, the wild-type and chimeric GPIX CHO cells gave only background signals (< 10% positive cells). Reactivity of patient sera with CHO cells expressing chimera 3 is substantially reduced.

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