Table 4.

Intraclonal heterogeneity of PIM-1, PAX-5, RhoH/TTF, and IgVH genes in AIDS-NHL

Sample
Histology
Gene
No. of mutations (position) in the tumor clone*
No. of DNA clones analyzed
Total no. of DNA clones with additional mutations†
Total no. of additional mutations
Total no. of bp sequenced
1138   AIDS-DLBCL  PIM-1  2 (1764G>A, 1765G>C)   34   2   2   24310  
   VH2 (S12-9)   97.8%   26   5   8   6900  
1220   AIDS-DLBCL  RhoH/TTF  2 (418Δ31 bp, 987T>C)   50   5   5   43750  
2378   AIDS-DLBCL  PIM-1  2 (1720C>T, 1721C>G)   65   27   34   46475  
   VH3 (DP-48/13)   96.7%   27   13   19   4140  
2256   AIDS-PCNSL  RhoH/TTF  5 (546G>A, 652A>C, 699T>C, 748A>T, 757A>G)   30   0   0   26250  
   VH4 (DP-63)   78.8%   20   11   25   7130  
645   AIDS-BL  RhoH/TTF  4 (557T>G, 563A>G, 638C>T, 643A>G)   25   0   0   21875  
1248   AIDS-BL  PIM-1  1 (1496G>A)   26   1   1   18590  
   VH4 (DP-66)   91.4%   17   5   5   3910  
2106
 		 AIDS-BL
 		PAX-5
 		 1 (1394C>T)
 		 25
 		 0
 		 0
 		 23300
 
Sample
Histology
Gene
No. of mutations (position) in the tumor clone*
No. of DNA clones analyzed
Total no. of DNA clones with additional mutations†
Total no. of additional mutations
Total no. of bp sequenced
1138   AIDS-DLBCL  PIM-1  2 (1764G>A, 1765G>C)   34   2   2   24310  
   VH2 (S12-9)   97.8%   26   5   8   6900  
1220   AIDS-DLBCL  RhoH/TTF  2 (418Δ31 bp, 987T>C)   50   5   5   43750  
2378   AIDS-DLBCL  PIM-1  2 (1720C>T, 1721C>G)   65   27   34   46475  
   VH3 (DP-48/13)   96.7%   27   13   19   4140  
2256   AIDS-PCNSL  RhoH/TTF  5 (546G>A, 652A>C, 699T>C, 748A>T, 757A>G)   30   0   0   26250  
   VH4 (DP-63)   78.8%   20   11   25   7130  
645   AIDS-BL  RhoH/TTF  4 (557T>G, 563A>G, 638C>T, 643A>G)   25   0   0   21875  
1248   AIDS-BL  PIM-1  1 (1496G>A)   26   1   1   18590  
   VH4 (DP-66)   91.4%   17   5   5   3910  
2106
 		 AIDS-BL
 		PAX-5
 		 1 (1394C>T)
 		 25
 		 0
 		 0
 		 23300
 
*

Mutations detected by DNA direct sequencing of the PCR product. For the IgVH genes, the percentage of homology to the closest germ line gene is given.

†

Number of DNA clones containing mutations that are not visible on direct sequencing and may, therefore, represent subclonal variants of the tumor.

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