Table 1.

Enumeration of total, T, and B cells in the various organs of wild-type and B7-H2–deficient mice



+/+

-/-
Cell type
Total cells, × 107
CD3+, × 107
IgM+ B220+, × 107
Total cells, × 107
CD3+, × 107
IgM+B220+, × 107
Thymus   7.05 ± 1.61   6.04 ± 2.31   NA   9.23 ± 1.69   7.74 ± 2.84   NA  
Spleen   10.20 ± 0.56   2.18 ± 0.12   5.35 ± 0.59   13.46 ± 2.03   1.96 ± 0.42   5.37 ± 1.16  
Lymph node   1.78 ± 0.31   1.14 ± 0.23   0.38 ± 0.11   1.36 ± 0.23   0.74 ± 0.04   0.26 ± 0.07  
Bone marrow   2.22 ± 0.22   NA   0.52 ± 0.08   2.50 ± 0.24   NA   0.42 ± 0.11  
Peritoneum
 
0.67 ± 0.11
 
0.03 ± 0.01
 
0.20 ± 0.04
 
0.75 ± 0.05
 
0.03 ± 0.01
 
0.21 ± 0.06
 


+/+

-/-
Cell type
Total cells, × 107
CD3+, × 107
IgM+ B220+, × 107
Total cells, × 107
CD3+, × 107
IgM+B220+, × 107
Thymus   7.05 ± 1.61   6.04 ± 2.31   NA   9.23 ± 1.69   7.74 ± 2.84   NA  
Spleen   10.20 ± 0.56   2.18 ± 0.12   5.35 ± 0.59   13.46 ± 2.03   1.96 ± 0.42   5.37 ± 1.16  
Lymph node   1.78 ± 0.31   1.14 ± 0.23   0.38 ± 0.11   1.36 ± 0.23   0.74 ± 0.04   0.26 ± 0.07  
Bone marrow   2.22 ± 0.22   NA   0.52 ± 0.08   2.50 ± 0.24   NA   0.42 ± 0.11  
Peritoneum
 
0.67 ± 0.11
 
0.03 ± 0.01
 
0.20 ± 0.04
 
0.75 ± 0.05
 
0.03 ± 0.01
 
0.21 ± 0.06
 

Calculations of T and B cells are based on the total cell count and the percentage of CD3+ and IgM+B220+ cells as assayed by FACS staining. Cell counts are presented as mean ± SEM. Sample size in each group is at least 3. NA indicates not applicable.

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