Enumeration of total, T, and B cells in the various organs of wild-type and B7-H2–deficient mice
. | +/+ . | . | . | -/- . | . | . | ||||
---|---|---|---|---|---|---|---|---|---|---|
Cell type . | Total cells, × 107 . | CD3+, × 107 . | IgM+ B220+, × 107 . | Total cells, × 107 . | CD3+, × 107 . | IgM+B220+, × 107 . | ||||
Thymus | 7.05 ± 1.61 | 6.04 ± 2.31 | NA | 9.23 ± 1.69 | 7.74 ± 2.84 | NA | ||||
Spleen | 10.20 ± 0.56 | 2.18 ± 0.12 | 5.35 ± 0.59 | 13.46 ± 2.03 | 1.96 ± 0.42 | 5.37 ± 1.16 | ||||
Lymph node | 1.78 ± 0.31 | 1.14 ± 0.23 | 0.38 ± 0.11 | 1.36 ± 0.23 | 0.74 ± 0.04 | 0.26 ± 0.07 | ||||
Bone marrow | 2.22 ± 0.22 | NA | 0.52 ± 0.08 | 2.50 ± 0.24 | NA | 0.42 ± 0.11 | ||||
Peritoneum | 0.67 ± 0.11 | 0.03 ± 0.01 | 0.20 ± 0.04 | 0.75 ± 0.05 | 0.03 ± 0.01 | 0.21 ± 0.06 |
. | +/+ . | . | . | -/- . | . | . | ||||
---|---|---|---|---|---|---|---|---|---|---|
Cell type . | Total cells, × 107 . | CD3+, × 107 . | IgM+ B220+, × 107 . | Total cells, × 107 . | CD3+, × 107 . | IgM+B220+, × 107 . | ||||
Thymus | 7.05 ± 1.61 | 6.04 ± 2.31 | NA | 9.23 ± 1.69 | 7.74 ± 2.84 | NA | ||||
Spleen | 10.20 ± 0.56 | 2.18 ± 0.12 | 5.35 ± 0.59 | 13.46 ± 2.03 | 1.96 ± 0.42 | 5.37 ± 1.16 | ||||
Lymph node | 1.78 ± 0.31 | 1.14 ± 0.23 | 0.38 ± 0.11 | 1.36 ± 0.23 | 0.74 ± 0.04 | 0.26 ± 0.07 | ||||
Bone marrow | 2.22 ± 0.22 | NA | 0.52 ± 0.08 | 2.50 ± 0.24 | NA | 0.42 ± 0.11 | ||||
Peritoneum | 0.67 ± 0.11 | 0.03 ± 0.01 | 0.20 ± 0.04 | 0.75 ± 0.05 | 0.03 ± 0.01 | 0.21 ± 0.06 |
Calculations of T and B cells are based on the total cell count and the percentage of CD3+ and IgM+B220+ cells as assayed by FACS staining. Cell counts are presented as mean ± SEM. Sample size in each group is at least 3. NA indicates not applicable.