Induction of HbF in erythroid precursors from thalassemia patients
. | . | . | Mithramycin, nM . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|---|
| Patient . | Untreated . | HU, 125 μM . | 10 . | 15 . | 20 . | 25 . | |||
| 1 | |||||||||
| Cells* | 4.3 | 0.88 | 3.6 | 4.8 | 4.2 | 3.4 | |||
| %HbF | 3.6 | 13.6 | 10.3 | n.d. | 12.8 | 14.2 | |||
| 2 | |||||||||
| Cells | 3.9 | 0.7 | 3.5 | 3.8 | 3.6 | 3.1 | |||
| %HbF | 10.7 | 17.1 | 15.6 | 12.6 | 18.1 | 12.6 | |||
| 3 | |||||||||
| Cells | 4 | 3.6 | 3.1 | 3.3 | 2.8 | 2.3 | |||
| %HbF | 14 | 13.5 | 23.8 | 13.6 | 28.9 | 26.8 | |||
| 4 | |||||||||
| Cells | 1.3 | toxic | 1.5 | n.d. | 0.8 | n.d. | |||
| %HbF | 20 | toxic | 25.2 | n.d. | 44.7 | n.d. | |||
| 5 | |||||||||
| Cells | 3.6 | 0.7 | 2.7 | 3 | 2.6 | 1.8 | |||
| %HbF | 26.3 | 37.4 | 21.4 | 31.1 | 35.8 | 50 | |||
| 6 | |||||||||
| Cells | 1.53 | 0.26 | n.d. | 0.9 | 1.3 | 0.9 | |||
| %HbF | 28 | 23 | n.d. | 29.2 | 25.7 | 32.2 | |||
| 7 | |||||||||
| Cells | 0.86 | 0.15 | n.d. | 0.8 | 0.6 | 0.1 | |||
| %HbF | 56.1 | 61.6 | n.d. | 71.6 | 64.9 | n.d. | |||
| 8 | |||||||||
| Cells | 1.7 | 0.6 | n.d. | 1.8 | 1.9 | 1.1 | |||
| %HbF | 73.2 | 78.1 | n.d. | 75.8 | 76.4 | 80 | |||
| 9 | |||||||||
| Cells | 1.1 | 0.4 | n.d. | 0.8 | n.d. | 0.5 | |||
| %HbF | 79.8 | 88 | n.d. | 95.4 | n.d. | 84.3 | |||
. | . | . | Mithramycin, nM . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|---|
| Patient . | Untreated . | HU, 125 μM . | 10 . | 15 . | 20 . | 25 . | |||
| 1 | |||||||||
| Cells* | 4.3 | 0.88 | 3.6 | 4.8 | 4.2 | 3.4 | |||
| %HbF | 3.6 | 13.6 | 10.3 | n.d. | 12.8 | 14.2 | |||
| 2 | |||||||||
| Cells | 3.9 | 0.7 | 3.5 | 3.8 | 3.6 | 3.1 | |||
| %HbF | 10.7 | 17.1 | 15.6 | 12.6 | 18.1 | 12.6 | |||
| 3 | |||||||||
| Cells | 4 | 3.6 | 3.1 | 3.3 | 2.8 | 2.3 | |||
| %HbF | 14 | 13.5 | 23.8 | 13.6 | 28.9 | 26.8 | |||
| 4 | |||||||||
| Cells | 1.3 | toxic | 1.5 | n.d. | 0.8 | n.d. | |||
| %HbF | 20 | toxic | 25.2 | n.d. | 44.7 | n.d. | |||
| 5 | |||||||||
| Cells | 3.6 | 0.7 | 2.7 | 3 | 2.6 | 1.8 | |||
| %HbF | 26.3 | 37.4 | 21.4 | 31.1 | 35.8 | 50 | |||
| 6 | |||||||||
| Cells | 1.53 | 0.26 | n.d. | 0.9 | 1.3 | 0.9 | |||
| %HbF | 28 | 23 | n.d. | 29.2 | 25.7 | 32.2 | |||
| 7 | |||||||||
| Cells | 0.86 | 0.15 | n.d. | 0.8 | 0.6 | 0.1 | |||
| %HbF | 56.1 | 61.6 | n.d. | 71.6 | 64.9 | n.d. | |||
| 8 | |||||||||
| Cells | 1.7 | 0.6 | n.d. | 1.8 | 1.9 | 1.1 | |||
| %HbF | 73.2 | 78.1 | n.d. | 75.8 | 76.4 | 80 | |||
| 9 | |||||||||
| Cells | 1.1 | 0.4 | n.d. | 0.8 | n.d. | 0.5 | |||
| %HbF | 79.8 | 88 | n.d. | 95.4 | n.d. | 84.3 | |||
n.d. indicates not done.
Cell number per milliliter of culture × 10-6.