Patterns of VDJ junctions cloned from heterozygous aΔhs3b/hs4/bwt mice
. | Wild type . | Δhs3b/hs4 . |
|---|---|---|
| Number of sequences analyzed | 50 | 55 |
| CDR3 length | 35.9 ± 8.0 | 35.0 ± 6.7 |
| Total N nucleotides (VD + DJ) | 9.0 ± 4.2 | 9.5 ± 4.3 |
| PNAhigh, % functional sequences (functional sequences/total) | 86.4 (22/26) | 14.3 (5/28) |
| PNAlow, % functional sequences (functional sequences/total) | 41.7 (10/24) | 40.7 (11/27) |
| D usage, % | ||
| Dsp2,1 | 5.1 | 3.9 |
| Dsp2,2 | 5.1 | 15.7 |
| Dsp2.3 | 0 | 3.9 |
| Dsp2.4 | 0 | 2 |
| Dsp2.5 | 7.7 | 13.7 |
| Dsp2.7 | 2.6 | 3.9 |
| Dsp2,9 | 12.8 | 7.8 |
| Dsp2,x | 7.7 | 0 |
| Dfl16,1 | 30.8 | 35.3 |
| Dfl16.2 | 0 | 2 |
| DQ52 | 17.9 | 5.9 |
| Undetermined | 10.2 | 5.9 |
. | Wild type . | Δhs3b/hs4 . |
|---|---|---|
| Number of sequences analyzed | 50 | 55 |
| CDR3 length | 35.9 ± 8.0 | 35.0 ± 6.7 |
| Total N nucleotides (VD + DJ) | 9.0 ± 4.2 | 9.5 ± 4.3 |
| PNAhigh, % functional sequences (functional sequences/total) | 86.4 (22/26) | 14.3 (5/28) |
| PNAlow, % functional sequences (functional sequences/total) | 41.7 (10/24) | 40.7 (11/27) |
| D usage, % | ||
| Dsp2,1 | 5.1 | 3.9 |
| Dsp2,2 | 5.1 | 15.7 |
| Dsp2.3 | 0 | 3.9 |
| Dsp2.4 | 0 | 2 |
| Dsp2.5 | 7.7 | 13.7 |
| Dsp2.7 | 2.6 | 3.9 |
| Dsp2,9 | 12.8 | 7.8 |
| Dsp2,x | 7.7 | 0 |
| Dfl16,1 | 30.8 | 35.3 |
| Dfl16.2 | 0 | 2 |
| DQ52 | 17.9 | 5.9 |
| Undetermined | 10.2 | 5.9 |