Phenotype of undifferentiated hESCs and EB-derived cells differentiated in the presence of extrinsic factors for 15 days
. | % of live (7-AAD-) cells expressing hematopoietic markers . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|
| . | . | Differentiated hESCs (EBs) . | . | . | . | ||||
| Phenotype . | Undifferentiated hESCs . | Control . | Cytokines . | Cytokines + BMP-4 . | BMP-4 . | ||||
| c-Kit+ | 32.0±9.2* | 7.6±1.5 | 6.3±2.6 | 9.1±1.6 | 8.5±1.5 | ||||
| c-Kit+ CD34+ | 1.2±0.4* | 3.4±0.9 | 4.1±2.0 | 7.3±1.5† | 4.2±1.2 | ||||
| AC133+ | 25.0±8.2 | 25.5±3.2 | 18.9±4.5 | 19.9±3.0 | 27.5±5.9 | ||||
| AC133+ CD34- | 24.2±8.1 | 23.3±2.7 | 17.0±4.0 | 16.9±2.3 | 23.8±4.8 | ||||
. | % of live (7-AAD-) cells expressing hematopoietic markers . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|
| . | . | Differentiated hESCs (EBs) . | . | . | . | ||||
| Phenotype . | Undifferentiated hESCs . | Control . | Cytokines . | Cytokines + BMP-4 . | BMP-4 . | ||||
| c-Kit+ | 32.0±9.2* | 7.6±1.5 | 6.3±2.6 | 9.1±1.6 | 8.5±1.5 | ||||
| c-Kit+ CD34+ | 1.2±0.4* | 3.4±0.9 | 4.1±2.0 | 7.3±1.5† | 4.2±1.2 | ||||
| AC133+ | 25.0±8.2 | 25.5±3.2 | 18.9±4.5 | 19.9±3.0 | 27.5±5.9 | ||||
| AC133+ CD34- | 24.2±8.1 | 23.3±2.7 | 17.0±4.0 | 16.9±2.3 | 23.8±4.8 | ||||
Values represent the means ± SEM from 4-8 independent experiments. BMP-4 indicates bone morphogenetic protein-4
Significant (P < .01) difference from differentiated hESCs in all treatment based on the cell surface phenotype specified
Significant difference (P < .01) from all other treatments of differentiated hESCs