Primer and probe combinations used in the quantitative RT-PCR
Target cDNA . | Primer/probe combinations . | . | |
|---|---|---|---|
| Pgp | Forward | 5′-CAGCTCCTGGAGCGGTTCTA | |
| Reverse | 5′-CGCTTTATTTCTTTGCCATCAA | ||
| Probe | 5′-CCCCTTGGCAGGGAAAGTGCTGC | ||
| PCR product size | 68 bp | ||
| MRP1 | Forward | 5′-TCATGGTGCCCGTCAATG | |
| Reverse | 5′-CGATTGTCTTTGCTCTTCATGTG | ||
| Probe | 5′-ATGGCGATGAAGACCAAGACGTATCAGGT | ||
| PCR product size | 79 bp | ||
| MRP2 | Forward | 5′-TGCAGCCTCCATAACCATGA | |
| Reverse | 5′-GGACTTCAGATGCCTGCCA | ||
| Probe | 5′-TCGAACACTTAGCCGCAGTTCTAGGTCCA | ||
| PCR product size | 88 bp | ||
| β2-microglobulin | Forward | 5′-CATTCGGGCCGAGATGTC | |
| Reverse | 5′-CTCCAGGCCAGAAAGAGAGAGTAG | ||
| Probe | 5′-CCGTGGCCTTAGCTGTGCTCGC | ||
| PCR product size | 70 bp | ||
Target cDNA . | Primer/probe combinations . | . | |
|---|---|---|---|
| Pgp | Forward | 5′-CAGCTCCTGGAGCGGTTCTA | |
| Reverse | 5′-CGCTTTATTTCTTTGCCATCAA | ||
| Probe | 5′-CCCCTTGGCAGGGAAAGTGCTGC | ||
| PCR product size | 68 bp | ||
| MRP1 | Forward | 5′-TCATGGTGCCCGTCAATG | |
| Reverse | 5′-CGATTGTCTTTGCTCTTCATGTG | ||
| Probe | 5′-ATGGCGATGAAGACCAAGACGTATCAGGT | ||
| PCR product size | 79 bp | ||
| MRP2 | Forward | 5′-TGCAGCCTCCATAACCATGA | |
| Reverse | 5′-GGACTTCAGATGCCTGCCA | ||
| Probe | 5′-TCGAACACTTAGCCGCAGTTCTAGGTCCA | ||
| PCR product size | 88 bp | ||
| β2-microglobulin | Forward | 5′-CATTCGGGCCGAGATGTC | |
| Reverse | 5′-CTCCAGGCCAGAAAGAGAGAGTAG | ||
| Probe | 5′-CCGTGGCCTTAGCTGTGCTCGC | ||
| PCR product size | 70 bp | ||