Hematopoietic colonies derived from GFP-expressing cells
. | . | . | . | No. of colonies/10 000 cells . | . | |
|---|---|---|---|---|---|---|
| Samples (line no.) . | Region . | Fraction . | Percentage . | Day 4 . | Day 7 . | |
| E11.5 (685) | AGM | Total | 100 | < 5 | < 5 | |
| E11.5 (685) | AGM | TG-GFP+/CD45+ | 0.09 | 6100 | ++ | |
| E11.5 (685) | AGM | TG-GFP+/CD45- | 1.97 | 180 | 550 | |
| E11.5 (798) | AGM | Total | 100 | 0 | 15 | |
| E11.5 (798) | AGM | TG-GFP+/CD45+ | 0.09 | 4200 | 3700 | |
| E11.5 (798) | AGM | TG-GFP+/CD45- | 1.58 | 120 | 1400 | |
| E10.5 (685) | AGM | Total | 100 | 16 | 70 | |
| E10.5 (685) | AGM | TG-GFP+/CD45+ | 0.05 | 700 | 2400 | |
| E10.5 (685) | AGM | TG-GFP+/CD45- | 2.08 | 700 | 3550 | |
| E10.5 (798) | AGM | Total | 100 | 13 | 80 | |
| E10.5 (798) | AGM | TG-GFP+/CD45+ | 0.02 | 4000 | ++ | |
| E10.5 (798) | AGM | TG-GFP+/CD45- | 0.37 | 635 | ++ | |
| E9.5 (685) | P-Sp | Total | 100 | < 1 | < 1 | |
| E9.5 (685) | P-Sp | TG-GFP+/CD45+ | 0.38 | < 50 | < 50 | |
| E9.5 (685) | P-Sp | TG-GFP+/CD45- | 0.59 | 5 | 15 | |
| E9.5 (798) | P-Sp | Total | 100 | < 1 | < 1 | |
| E9.5 (798) | P-Sp | TG-GFP+/CD45+ | 0.06 | < 50 | 750 | |
| E9.5 (798) | P-Sp | TG-GFP+/CD45- | 0.46 | 65 | 235 | |
| E9.5 (685) | YS | Total | 100 | 23 | < 1 | |
| E9.5 (685) | YS | TG-GFP+/CD45+ | 0.28 | 100 | 750 | |
| E9.5 (685) | YS | TG-GFP+/CD45- | 1.77 | 10 | < 5 | |
| E9.5 (798) | YS | Total | 100 | 19 | 6 | |
| E9.5 (798) | YS | TG-GFP+/CD45+ | 0.14 | 10 | 300 | |
| E9.5 (798) | YS | TG-GFP+/CD45- | 2.30 | < 5 | < 5 | |
. | . | . | . | No. of colonies/10 000 cells . | . | |
|---|---|---|---|---|---|---|
| Samples (line no.) . | Region . | Fraction . | Percentage . | Day 4 . | Day 7 . | |
| E11.5 (685) | AGM | Total | 100 | < 5 | < 5 | |
| E11.5 (685) | AGM | TG-GFP+/CD45+ | 0.09 | 6100 | ++ | |
| E11.5 (685) | AGM | TG-GFP+/CD45- | 1.97 | 180 | 550 | |
| E11.5 (798) | AGM | Total | 100 | 0 | 15 | |
| E11.5 (798) | AGM | TG-GFP+/CD45+ | 0.09 | 4200 | 3700 | |
| E11.5 (798) | AGM | TG-GFP+/CD45- | 1.58 | 120 | 1400 | |
| E10.5 (685) | AGM | Total | 100 | 16 | 70 | |
| E10.5 (685) | AGM | TG-GFP+/CD45+ | 0.05 | 700 | 2400 | |
| E10.5 (685) | AGM | TG-GFP+/CD45- | 2.08 | 700 | 3550 | |
| E10.5 (798) | AGM | Total | 100 | 13 | 80 | |
| E10.5 (798) | AGM | TG-GFP+/CD45+ | 0.02 | 4000 | ++ | |
| E10.5 (798) | AGM | TG-GFP+/CD45- | 0.37 | 635 | ++ | |
| E9.5 (685) | P-Sp | Total | 100 | < 1 | < 1 | |
| E9.5 (685) | P-Sp | TG-GFP+/CD45+ | 0.38 | < 50 | < 50 | |
| E9.5 (685) | P-Sp | TG-GFP+/CD45- | 0.59 | 5 | 15 | |
| E9.5 (798) | P-Sp | Total | 100 | < 1 | < 1 | |
| E9.5 (798) | P-Sp | TG-GFP+/CD45+ | 0.06 | < 50 | 750 | |
| E9.5 (798) | P-Sp | TG-GFP+/CD45- | 0.46 | 65 | 235 | |
| E9.5 (685) | YS | Total | 100 | 23 | < 1 | |
| E9.5 (685) | YS | TG-GFP+/CD45+ | 0.28 | 100 | 750 | |
| E9.5 (685) | YS | TG-GFP+/CD45- | 1.77 | 10 | < 5 | |
| E9.5 (798) | YS | Total | 100 | 19 | 6 | |
| E9.5 (798) | YS | TG-GFP+/CD45+ | 0.14 | 10 | 300 | |
| E9.5 (798) | YS | TG-GFP+/CD45- | 2.30 | < 5 | < 5 | |
++ indicates numerous; P-Sp, para-aortic splanchno-pleura; and YS, yolk sac