Cell cycle analysis of L540cy HD cells
. | Sub-G1 R1 . | G1 R2 . | S-phase R3 . | G2 R4 . | Sub-G2 R5 . |
|---|---|---|---|---|---|
| 12 h | |||||
| Untreated | 2.0 | 47.9 | 37.6 | 9.7 | 1.9 |
| cAC10 | 2.2 | 35.3 | 52.3 | 7.5 | 1.3 |
| cAC10-vcMMAE | 2.5 | 5.7 | 36.5 | 46.5 | 2.5 |
| clgG-vcMMAE | 1.6 | 36.1 | 46.4 | 12.3 | 2.1 |
| 24 h | |||||
| Untreated | 1.6 | 38.5 | 47.4 | 7.7 | 4.8 |
| cAC10 | 1.9 | 39.3 | 44.8 | 11.8 | 2.2 |
| cAC10-vcMMAE | 19.2 | 10.2 | 27.8 | 28.3 | 6.6 |
| clgG-vcMMAE | 4.6 | 31.5 | 38.5 | 18.9 | 3.2 |
| 48 h | |||||
| Untreated | 1.9 | 43.7 | 39.3 | 11.6 | 3.1 |
| cAC10 | 2.8 | 46.7 | 40.5 | 0.4 | 10.4 |
| cAC10-vcMMAE | 53.1 | 1.5 | 26.3 | 0.9 | 1.0 |
| clgG-vcMMAE | 5.5 | 49.0 | 26.9 | 11.9 | 6.1 |
. | Sub-G1 R1 . | G1 R2 . | S-phase R3 . | G2 R4 . | Sub-G2 R5 . |
|---|---|---|---|---|---|
| 12 h | |||||
| Untreated | 2.0 | 47.9 | 37.6 | 9.7 | 1.9 |
| cAC10 | 2.2 | 35.3 | 52.3 | 7.5 | 1.3 |
| cAC10-vcMMAE | 2.5 | 5.7 | 36.5 | 46.5 | 2.5 |
| clgG-vcMMAE | 1.6 | 36.1 | 46.4 | 12.3 | 2.1 |
| 24 h | |||||
| Untreated | 1.6 | 38.5 | 47.4 | 7.7 | 4.8 |
| cAC10 | 1.9 | 39.3 | 44.8 | 11.8 | 2.2 |
| cAC10-vcMMAE | 19.2 | 10.2 | 27.8 | 28.3 | 6.6 |
| clgG-vcMMAE | 4.6 | 31.5 | 38.5 | 18.9 | 3.2 |
| 48 h | |||||
| Untreated | 1.9 | 43.7 | 39.3 | 11.6 | 3.1 |
| cAC10 | 2.8 | 46.7 | 40.5 | 0.4 | 10.4 |
| cAC10-vcMMAE | 53.1 | 1.5 | 26.3 | 0.9 | 1.0 |
| clgG-vcMMAE | 5.5 | 49.0 | 26.9 | 11.9 | 6.1 |
Flow cytometry data are shown in Figure 5 and in comparable plots of untreated cells.
Cells treated with cAC10 were analyzed using CellQuest Software (Becton Dickinson) to determine the percentages of cells in regions R1 through R5.