Cytotoxicity of cAC10-vcMMAE and free MMAE on CD30+ and CD30– tumor lines
. | . | . | IC50 . | . | |
|---|---|---|---|---|---|
| Cell line . | Lineage . | Binding ratio . | cAC10-vcMMAE, ng/mL . | MMAE, nM . | |
| L540 | Hodgkin disease, T-cell like | 318 | 9.88 ± 2.49 (n=4) | 0.21 ± 0.12 (n = 3) | |
| Karpas | Anaplastic large cell lymphoma | 163 | 1.29 ± 0.46 (n=5) | 0.073 ± 0.05 (n = 5) | |
| HDLM2 | Hodgkin disease, T-cell like | 147 | 302 ± 177 (n=4) | 0.072 ± 0.03 (n = 4) | |
| HH | Cutaneous T-cell lymphoma | 73 | 2.0 ± 1.7 (n=3) | 0.27 ± 0.09 (n = 3) | |
| SU-DHL-1 | Anaplastic large cell lymphoma | 54 | 0.54 ± 0.19 (n=4) | 0.039 ± 0.015 (n = 4) | |
| Raji | Burkitt lymphoma B cell | 2 | 8701 ± 666 (n=3) | 0.14 ± 0.07 (n = 3) | |
| Ramos | Burkitt lymphoma B cell | 0.9 | 3333 ± 682 (n=3) | 0.039 ± 0.02 (n = 4) | |
| Daudi | Burkitt lymphoma B cell | 1 | 7500 ± 443 (n=3) | 1.35 ± 0.31 (n = 3) | |
. | . | . | IC50 . | . | |
|---|---|---|---|---|---|
| Cell line . | Lineage . | Binding ratio . | cAC10-vcMMAE, ng/mL . | MMAE, nM . | |
| L540 | Hodgkin disease, T-cell like | 318 | 9.88 ± 2.49 (n=4) | 0.21 ± 0.12 (n = 3) | |
| Karpas | Anaplastic large cell lymphoma | 163 | 1.29 ± 0.46 (n=5) | 0.073 ± 0.05 (n = 5) | |
| HDLM2 | Hodgkin disease, T-cell like | 147 | 302 ± 177 (n=4) | 0.072 ± 0.03 (n = 4) | |
| HH | Cutaneous T-cell lymphoma | 73 | 2.0 ± 1.7 (n=3) | 0.27 ± 0.09 (n = 3) | |
| SU-DHL-1 | Anaplastic large cell lymphoma | 54 | 0.54 ± 0.19 (n=4) | 0.039 ± 0.015 (n = 4) | |
| Raji | Burkitt lymphoma B cell | 2 | 8701 ± 666 (n=3) | 0.14 ± 0.07 (n = 3) | |
| Ramos | Burkitt lymphoma B cell | 0.9 | 3333 ± 682 (n=3) | 0.039 ± 0.02 (n = 4) | |
| Daudi | Burkitt lymphoma B cell | 1 | 7500 ± 443 (n=3) | 1.35 ± 0.31 (n = 3) | |
Lineages as listed in Gruss et al.22
Binding ratios were determined by dividing the geometric mean fluorescence intensity of cells stained with cAC10 and secondary (goat-antihuman immunoglobulin)-FITC conjugate by the geometric mean fluorescence intensity of cells stained with secondary antibody alone.
IC50 results are expressed as mean ± SEM.
Values in parentheses represent number of experiments, with each experimental result an average of quadruplicate determinations.