Table 3. Reduction of plasma... | American Society of Hematology

Table 3.

Reduction of plasma autoantibodies against GPIb/IX is associated with increased yield of CD41⁺PI⁺ cells

	Before absorption
	Process 1^*	Process 2†	After absorption
Acute ITP no. 1; Plt, 2 × 10³/μL
IgM titer	>160	>160	80
CD41⁺PI⁺, % of control	25	25	50
Acute ITP no. 2; Plt, 18 × 10³/μL
IgM titer	>20	>20	Undetectable
CD41⁺PI⁺, % of control	82	66	120
Healthy control no. 1; Plt, control
IgM titer	Undetectable	Undetectable	Undetectable
CD41⁺PI⁺, % of control	100	94	100
Healthy control no. 2; Plt, control
IgM titer	Undetectable	Undetectable	Undetectable
CD41⁺PI⁺, % of control	100	108	100

	Before absorption
	Process 1^*	Process 2†	After absorption
Acute ITP no. 1; Plt, 2 × 10³/μL
IgM titer	>160	>160	80
CD41⁺PI⁺, % of control	25	25	50
Acute ITP no. 2; Plt, 18 × 10³/μL
IgM titer	>20	>20	Undetectable
CD41⁺PI⁺, % of control	82	66	120
Healthy control no. 1; Plt, control
IgM titer	Undetectable	Undetectable	Undetectable
CD41⁺PI⁺, % of control	100	94	100
Healthy control no. 2; Plt, control
IgM titer	Undetectable	Undetectable	Undetectable
CD41⁺PI⁺, % of control	100	108	100

Frozen plasma samples were defrosted in ice, and analyzed immediately in culture for their effect on in vitro megakaryocytopoiesis

†

Frozen plasma samples were defrosted in ice, processed same as absorbed plasma samples except that no platelets were added to the plasma, and then analyzed in culture

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