Efficient multilineage reconstitution of NOD/SCID mice with CD34+flt3+ cells
Cell type . | No. cells . | Human engraftment at 6-8 wk, % (SEM) . | No mice positive for human myeloid and lymphoid reconstitution at 6-8 wk . | Human engraftment at 12-13 wk, % (SEM) . | No. mice positive for human myeloid and lymphoid reconstitution at 12-13 wk . |
|---|---|---|---|---|---|
| CD34+flt3+ | 50 000-70 000 | 19.7 (11.2)* | 13 of 16* | ND | ND |
| CD34+flt3- | 50 000-70 000 | 2.5 (2.3) | 7 of 17 | ND | ND |
| CD34+flt3+ | 2 500-5 000 | 3.9 (4.8)* | 13 of 18* | 3.0* (2.7) | 9 of 18* |
| CD34+flt3- | 5 000-10 000 | 0.13 (0.24) | 4 of 26 | 0.06 (0.14) | 2 of 26 |
Cell type . | No. cells . | Human engraftment at 6-8 wk, % (SEM) . | No mice positive for human myeloid and lymphoid reconstitution at 6-8 wk . | Human engraftment at 12-13 wk, % (SEM) . | No. mice positive for human myeloid and lymphoid reconstitution at 12-13 wk . |
|---|---|---|---|---|---|
| CD34+flt3+ | 50 000-70 000 | 19.7 (11.2)* | 13 of 16* | ND | ND |
| CD34+flt3- | 50 000-70 000 | 2.5 (2.3) | 7 of 17 | ND | ND |
| CD34+flt3+ | 2 500-5 000 | 3.9 (4.8)* | 13 of 18* | 3.0* (2.7) | 9 of 18* |
| CD34+flt3- | 5 000-10 000 | 0.13 (0.24) | 4 of 26 | 0.06 (0.14) | 2 of 26 |
Sorted CD34+flt3+ and CD34+flt3- cells (2 500-70 000) from CB were transplanted into irradiated NOD/SCID mice. Human multilineage reconstitution was evaluated by FACS at 6 to 8 and at 12 to 13 weeks after transplantation (as indicated in “Materials and methods”). Data are expressed as mean (SEM) percentage human reconstitution from 3 to 5 experiments for each experimental group. Mice were defined as positive when reconstitution of both lymphoid and myeloid human cells was detected in the BM (“Materials and methods”)
P <.05 when comparing level of reconstitution observed in mice that underwent transplantation with CD34+flt3+ and CD34+flt3- cells or in fraction of mice positive for human lymphomyeloid reconstitution after transplantation of the 2 populations. ND indicates not determined